Pumping Machinery
Mostrando 1-8 de 8 artigos, teses e dissertações.
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1. Modelo hibrido para o estabelecimento de rotações otimas de bombas de rotação variavel
Computational simulations are, nowadays, tools that are present in the analysis process of hydraulic networks. The present dissertation brings the development of a computational model, to simulate, in extensive period, water supply systems operating with variable pumps speed, aiming at the optimum speeds of rotation that satisfy pre determined purposes, in t
Publicado em: 2002
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2. Evaluation of a class III biological safety cabinet for enclosure of an ultracentrifuge.
An evaluation of a special safety cabinet housing a high-speed centrifuge was made. The cabinet enclosed both the top access port and the drive and pumping machinery of the centrifuge. A titanium rotor was loaded with tubes containing a bacterial culture, weakened, and driven until rotor rupture occurred. There were several bent and broken components in the
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3. On the role of the K-proton transfer pathway in cytochrome c oxidase
Cytochrome c oxidase is a membrane-bound enzyme that catalyzes the four-electron reduction of oxygen to water. This highly exergonic reaction drives proton pumping across the membrane. One of the key questions associated with the function of cytochrome c oxidase is how the transfer of electrons and protons is coupled and how proton transfer is controlle
The National Academy of Sciences.
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4. Immunolocalization of the Plasma Membrane H+ -ATPase in Minor Veins of Vicia faba in Relation to Phloem Loading.
The immunolocalization of the plasma membrane H+ -ATPase, which generates a proton motive force energizing the uptake of inorganic and organic solutes, was studied by electron microscopy. The cells studied were in minor veins of Vicia faba L. exporting leaves, where photosynthates are supposed to be absorbed from the apoplast by phloem transfer cells. Immuno
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5. Fluorescence quenching of reconstituted NCD-4-labeled cytochrome c oxidase complex by DOXYL-stearic acids.
It has been known for some time that dicyclohexylcarbodiimide (DCCD) inhibits the proton translocation function of the cytochrome c oxidase complex (CcO) and that there is one major site in subunit III which is modified upon reaction with DCCD (Glu-90 for the bovine enzyme). We have examined the reaction of bovine CcO with N-cyclohexyl-N'-(4-dimethylamino-al
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6. Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue 1: I. Characterization of a Ca2+-Pumping ATPase Associated with the Endoplasmic Reticulum
Calcium transport was examined in microsomal membrane vesicles from red beet (Beta vulgaris L.) storage tissue using chlorotetracycline as a fluorescent probe. This probe demonstrates an increase in fluorescence corresponding to calcium accumulation within the vesicles which can be collapsed by the addition of the calcium ionophore A23187. Calcium uptake in
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7. ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance.
In Saccharomyces cerevisiae, 3-amino-1,2,4-triazole (aminotriazole) competitively inhibits the activity of imidazoleglycerolphosphate dehydratase, the product of the HIS3 gene. Wild-type strains are able to grow in the presence of 10 mM aminotriazole because they induce the level of imidazoleglycerolphosphate dehydratase. However, strains containing gcn4 mut
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8. In vivo modulation of nonphotochemical exciton quenching (NPQ) by regulation of the chloroplast ATP synthase
Nonphotochemical quenching (NPQ) of excitation energy, which protects higher plant photosynthetic machinery from photodamage, is triggered by acidification of the thylakoid lumen as a result of light-induced proton pumping, which also drives the synthesis of ATP. It is clear that the sensitivity of NPQ is modulated in response to changing physiological condi
The National Academy of Sciences.