Low Copy Number Dna
Mostrando 1-12 de 318 artigos, teses e dissertações.
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1. Sequence diversity and copy number variation of Mutator-like transposases in wheat
Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I and subclass II). The average pair-wise identity between members of both subclasses was 58.8
Genetics and Molecular Biology. Publicado em: 2008
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2. Desenvolvimento e aplicação de polimorfismos de inserção: deleção do cromossomo x em genética forense
Nowadays the human genetic identification its based on the utilization of a set of autosomal markers, the microsatellites, which fulfils the majority of the forensic genetic labs requirements. However, there are some situations when that the usual autosomal microsatellites dont always offer good results. Those situations include cases where the genetic sampl
Publicado em: 2007
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3. Estudo de dois grupos de elementos de cana-de-açúcar homológos à superfamília hAT de transposons / Studies on hAT-like transposases in sugarcane superfamily
Transposable elements (TEs) are mobile genetic sequences. Their mutagenic capacity makes them important sources of variation in the genomes. These elements have another important evolutionary role as donors of functional protein domains in the formation of new genes. 276 cDNA clones homologous to TEs were previously identified in the Brazilian Sugarcane Expr
Publicado em: 2007
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4. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research In
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-10
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5. Use of the TSPY gene for sexing cattle
The Y-encoded, testis-specific protein (TSPY) is a Y-specific gene. The copy numbers of TSPY range from 20 to 60 in men and up to 200 in bulls. In this study, we examined the possibility of using the TSPY gene to sex cattle. DNA from blood samples of 100 Nelore cattle (50 males and 50 females) from the Nelore Cattle Breeding Program (PMGRN) was screened for
Genetics and Molecular Biology. Publicado em: 2005-03
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6. Identificação e caracterização de proteinas que se associam in vitro a fita telomerica rica em G de Leishmania (Leishmania) amazonensis / Identification and characterization of proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand
The chromosomal ends of Leishmania (Leishmania) amazonensis contain conserved 5 - TTAGGG-3 telomeric repeats, that are maintained by telomerase. Using Telomeric Repeat Amplification Protocol (TRAP) we detected telomerase activity in protein fractions purified from S100 and nuclear extracts. Protein complexes that associate in vitro with telomeric DNA sequenc
Publicado em: 2004
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7. Genomic organization of low copy number sequences that are associated with deca-satellite DNA in the monkey genome.
A previously described segment of African green monkey DNA (cloned in phage lambda MkA) contains deca-satellite linked to DNA sequences that are estimated to occur once per genome. Sequences homologous to the low copy number sequences in lambda MkA are also associated with species-specific satellite DNAs in the human and mouse genomes. A second clone, lambda
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8. High-Resolution Analysis of DNA Copy Number Using Oligonucleotide Microarrays
Genomic copy number alterations are a feature of many human diseases including cancer. We have evaluated the effectiveness of an oligonucleotide array, originally designed to detect single-nucleotide polymorphisms, to assess DNA copy number. We first showed that fluorescent signal from the oligonucleotide array varies in proportion to both decreases and incr
Cold Spring Harbor Laboratory Press.
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9. Complementation of a bovine papilloma virus low-copy-number mutant: evidence for a temporal requirement of the complementing gene.
We identified a bovine papilloma virus function encoded by the E6/E7 gene, which is required for both BPV high-copy-number replication and maintenance of transformation of cultured cells. A cDNA copy of this gene was isolated and expressed from a retrovirus vector. We found that complete complementation of a BPV low-copy-number mutant (dl576) by the cDNA enc
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10. Microarray analysis reveals a major direct role of DNA copy number alteration in the transcriptional program of human breast tumors
Genomic DNA copy number alterations are key genetic events in the development and progression of human cancers. Here we report a genome-wide microarray comparative genomic hybridization (array CGH) analysis of DNA copy number variation in a series of primary human breast tumors. We have profiled DNA copy number alteration across 6,691 mapped human genes, in
The National Academy of Sciences.
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11. Quantifying single gene copy number by measuring fluorescent probe lengths on combed genomic DNA
An approach was developed for the quantification of subtle gains and losses of genomic DNA. The approach relies on a process called molecular combing. Molecular combing consists of the extension and alignment of purified molecules of genomic DNA on a glass coverslip. It has the advantage that a large number of genomes can be combed per coverslip, which allow
The National Academy of Sciences.
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12. Linear- and circular-plasmid copy numbers in Borrelia burgdorferi.
Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strain, B31, of B. burgdorferi. All three plas