Sequence diversity and copy number variation of Mutator-like transposases in wheat

AUTOR(ES)

Asakura, Nobuaki, Yoshida, Shinya, Mori, Naoki, Ohtsuka, Ichiro, Nakamura, Chiharu

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

2008

RESUMO

Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I and subclass II). The average pair-wise identity between members of both subclasses was 58.8% at the nucleotide sequence level. Sequence diversity of subclass I was larger than that of subclass II. DNA gel blot analysis showed that subclass I was present as low copy number elements in the genomes of all Triticum and Aegilops accessions surveyed, while subclass II was present as high copy number elements. These two subclasses seemed uncapable of recognizing each other for transposition. The number of copies of subclass II elements was much higher in Aegilops with the S, Sl and D genomes and polyploid Triticum species than in diploid Triticum with the A genome, indicating that active transposition occurred in S, Sl and D genomes before polyploidization. DNA gel blot analysis of six species selected from three subfamilies of Poaceae demonstrated that only the tribe Triticeae possessed both subclasses. These results suggest that the differentiation of these two subclasses occurred before or immediately after the establishment of the tribe Triticeae.

Documentos Relacionados

• Segmental Duplications and Copy-Number Variation in the Human Genome
• MSX2 copy number increase and craniosynostosis: copy number variation detected by array comparative genomic hybridization
• Parasite-host interaction in malaria: genetic clues and copy number variation
• Copy number variation influences gene expression and metabolic traits in mice
• Sequence similarity of putative transposases links the maize Mutator autonomous element and a group of bacterial insertion sequences.