Bioreactor Control
Mostrando 1-12 de 48 artigos, teses e dissertações.
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1. Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source
Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment a
Braz. J. Microbiol.. Publicado em: 2017-07
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2. BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION
Abstract Bioprocess development studies concerning the production of cellulases are of crucial importance due to the significant impact of these enzymes on the economics of biomass conversion into fuels and chemicals. This work evaluates the effects of solid-state fermentation (SSF) operational conditions on cellulase production by a novel strain of Aspergil
Braz. J. Chem. Eng.. Publicado em: 2016-03
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3. Polypropylene Composites Reinforced with Biodegraded Sugarcane Bagasse Fibers: Static and Dynamic Mechanical Properties
The present work aimed to study sugarcane bagasse fibers pre-treated with fungi and using NaOH/ anthraquinone (AQ) in chemical pulping processes for applications in composite materials. Bagasse was decayed with 250 mg of Ceriporiopsis subvermispora inoculum in a 20 L bioreactor. After that, samples were submitted to similar conditions of decaying without ino
Mat. Res.. Publicado em: 12/02/2016
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4. ON-LINE MONITORING OF BIOMASS CONCENTRATION BASED ON A CAPACITANCE SENSOR: ASSESSING THE METHODOLOGY FOR DIFFERENT BACTERIA AND YEAST HIGH CELL DENSITY FED-BATCH CULTURES
Abstract The performance of an in-situ capacitance sensor for on-line monitoring of biomass concentration was evaluated for some of the most important microorganisms in the biotechnology industry: Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Bacillus megaterium. A total of 33 batch and fed-batch cultures were carried out in a bench-scale b
Braz. J. Chem. Eng.. Publicado em: 2015-12
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5. BIOHYDROGEN FROM CHEESE WHEY TREATMENT IN AN AnSBBR: ACHIEVING PROCESS STABILITY
AbstractAn AnSBBR (anaerobic sequencing batch reactor containing biomass immobilized on an inert support) with liquid phase recirculation, containing a 3.5 L working volume, treated 1.5 L of cheese whey wastewater in 3 and 4 h cycles at 30 ºC to produce biohydrogen. From startup the bioreactor presented process instability. To overcome this problem the foll
Braz. J. Chem. Eng.. Publicado em: 2015-06
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6. Design of signal peptide bombyxin and its effect on secretory expression efficiency and levels of Helicobacter pylori urease subunit B in silkworm cells and larvae
This study employed a Bac-to-Bac/Bombyx mori bioreactor to mass-produce immunogenic urease subunit B (UreB) from Helicobacter pylori.The signal peptide bombyxin from B. mori was used to promote secretory expression to improve expression levels and was designed and integrated into the UreB gene to generate the Bacmid/BmNPV/(signal peptide)-UreB baculovirus ex
Braz. arch. biol. technol.. Publicado em: 2015-06
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7. Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil
Studies were conducted on the production of protease by moderately halophilic Bacillus sp. on agro-industrial waste materials. The bacterium could efficiently use many agro wastes as substrates but wheat bran supported maximum enzyme production. To ascertain the performance of the process in shake flasks and lab scale bioreactor, experiments were conducted t
Braz. arch. biol. technol.. Publicado em: 18/03/2014
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8. Influence of pectin, glucose, and pH on the production of endo- and exo-polygalacturonase by Aspergillus oryzae in liquid medium
Endo-and exo-polygalacturonase (PG) production by Aspergillus oryzae IPT 301 was evaluated in a batch bioreactor in media containing 0, 10, and 20 g.L-1 pectin and 0 to 30 g.L-1 glucose. For cultivations in which the pH was not controlled, the use of 20 g.L-1 pectin and 10 g.L-1 glucose resulted in superior enzyme activities compared to control media with 0
Braz. J. Chem. Eng.. Publicado em: 2012-12
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9. Molecular characterisation and biomass and metabolite production of Lactobacillus reuteri LPB P01-001: a potential probiotic
Lactobacillus reuteri LPB P01-001 was isolated from the gastrointestinal tract of wild swine and was characterised by biochemical testing and sequencing of gene 16S rRNA. A simple and low-cost culture medium based on cane sugar (2.5% p/v) and yeast extract (1% p/v) was used in the production of this probiotic. The fermentative conditions were a) pH control a
Brazilian Journal of Microbiology. Publicado em: 2012-03
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10. Sistema automático de supervisão e controle de cultivos de alta densidade celular de E. coli recombinante
Cultivos de alta densidade celular de E. coli recombinante constituem uma tecnica rapida e economica para producao de proteinas recombinantes. Por meio deste bioprocesso, sao obtidos produtos de alto valor agregado e de grande importancia na industria farmaceutica, tais como insulina, hormonios de crescimento humano e bovino, antigenos proteicos para formula
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/12/2011
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11. Modelos para a produção de eritropoietina recombinante humana in vivo e in vitro com vetores plasmideais em ovinos / Models for the production of human recombinant erythropoietin in vivo and in vitro with plasmidial vectors in ovine
Some post-translational modifications are necessary for the production of biopharmaceutical proteins, such as recombinant human erythropoietin (rhEPO), with a good specific action and a high biological activity. These modifications are obtained only by bioreactors based on eukaryotic cell as mammary cells. Bioreactors, in vivo or in vitro, with this kind of
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/02/2011
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12. Application of response surface methodology to enhancement of biomass production by Lactobacillus rhamnosus E/N
Response surface methodology (RSM) was employed to study the effects of various medium components on biomass production by Lactobacillus rhamnosus E/N. This strain is commonly used in the pharmaceutical and food industries due to its beneficial effect on the human gut and general health. The best medium composition derived from RSM regression was (in g/l) gl
Braz. J. Microbiol.. Publicado em: 2011-12