Influence of pectin, glucose, and pH on the production of endo- and exo-polygalacturonase by Aspergillus oryzae in liquid medium

AUTOR(ES)

Fontana, R. C., Silveira, M. M.

FONTE

Braz. J. Chem. Eng.

DATA DE PUBLICAÇÃO

2012-12

RESUMO

Endo-and exo-polygalacturonase (PG) production by Aspergillus oryzae IPT 301 was evaluated in a batch bioreactor in media containing 0, 10, and 20 g.L-1 pectin and 0 to 30 g.L-1 glucose. For cultivations in which the pH was not controlled, the use of 20 g.L-1 pectin and 10 g.L-1 glucose resulted in superior enzyme activities compared to control media with 0 and 10 g.L-1 of the inducer and the same glucose concentration. Maximum endo-PG and exo-PG activities were 125.0 and 76.3 U.mL-1, respectively. For cultivations in which the pH was controlled to a minimum value of 2.7, media containing 20 g.L-1 pectin and 20 to 50 g.L-1 glucose were tested and significant improvement of both activities was attained. Maximum enzyme activities (175.0 U.mL-1 for endo-PG and 103.0 U.mL-1 for exo-PG) were obtained in pH-controlled batch experiments with 30 g.L-1 glucose.

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