Extraction, stability and qualification of anthocyanins of Brazilian typical fruits for industrial application as colorants / Extração, estabilidade e quantificação de antocianinas de frutas tipicas brasileiras para aplicação industrial como corantes

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Anthocyanins (ACYS) are pigments found in plants that give colors from red to blue to flowers, fruits and leaves. The proposal of analytical studies involving extraction, stability and quantification of ACYS from different fruits takes into account the new perspectives of using these pigments in several segments of the industry, such as, pharmaceutical, cosmetic and food. In this work, extracts of 3 Brazilian typical frozen fruits: jaboticaba (Myrciaria culiflora), mulberry (Morus nigra) and blackberry (Rubus sp), were studied aiming to maximize the amount of extracted ACYS and to obtain extracts with longer durability. The studied conditions were: type of solvent, addition of preservatives in aqueous solvent, temperature of extraction and storage and storage time. The influence in extraction and stability of the extracts using the whole fruit was studied for jaboticaba. Ethanol 94% v/v was the extractor solvent which resulted in greater capacity of extraction and affered the most stable extracts. The mixture of Nipagin 0,01 % w/v + Nipazol 0,05 % w/v preservatives provided the best results, reducing the degradation of ACYS and preventing the fungi emergence. At optimized conditions, the levels of ACYS in the fruits, expressed in mg ACYS / 100 g were: 40,0±0,2 in jabuticaba, 153±3 in mulberry and 83±2 in blackberry. Comparative studies of different procedures for quantification of ACYS, including the official method and the use the ACYS commercial standards were done, including simplifications to be used in industrial routine. The results showed that the method which uses measurements of absorbance of extracts at pH 1,0 and at the wavelength of maximum absorption of ACYS allows easily quantify total ACYS without necessity of using commercial standard

ASSUNTO(S)

brazilian typical fruits antocianina anthocyanin corantes frutas tipicas brasileiras colorants

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