Clostridium estertheticum in raw milk and Parmesan cheese and provolen / Clostridium estertheticum em leite cru e em queijos parmesão e provolen

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The spoilage of semicooked and cooked cheeses and semihard and hard cheeses, known as late blowing, has been the focus of some studies. Some microorganisms already had been related with this spoilage including some species of Clostridium, as C. tyrobutiricum, C. butyricum, C. sporogenes and C. beijerinckii. A new species, C. estertheticum, is being incriminated in the spoilage of cooled meats packed the vacuum, which presents similar characteristics to the late blowing in cheeses, as gas production with blowing of the packing, fort flavor of rancid and presence of acid butyl. However, did not have a story on the occurrence of C. estertheticum in raw milk and cheeses. The present work evaluated 32 samples of raw milk proceeding from country properties of Goiás and 95 samples of cheeses commercialized parmesan and provolone in the retail market of Goiânia, produced in different States of Brazil. C. estertheticum was detected using of the Polimerase Chain Reaction with two pairs of primers, RF/RR and 16SEF/16SER. The effect of the not-selective enrichment of the samples in broth BHI was evaluated, in three incubation periods, five, ten and 30 days 10C in anaerobes. The genomics DNA was extracted following the methodology fenol:clorofórmio. The gotten results disclose to the occurrence of C. estertheticum in samples of raw milk (34,4%) and samples of cheeses parmesan and provolone (17,9%). The highest leves of C. estertheticum was gotten in the cheeses parmesan and provolone with signals of spoilage (50,0%) and normal (29,6%) and in provolone with spoilage signals (25,0%). C. estertheticum was detected with the two pairs of primers used in the amplification, being the totality of positives only gotten when it associated the two pairs. The daily pay-enrichment for extraction of the genomics DNA substantially influenced in the detention of the microorganism being the period of 10 days, what it provided better resulted for pair RF/RR. C. estertheticum was found in samples of cheeses proceeding from all the analyzed States, and in raw milk samples harvested in Goiás, what it indicates its dissemination in Brazil

ASSUNTO(S)

produtos lácteos, deterioração de queijo, pcr, clostridium dairy products, spoilage of cheese, pcr, clostridium tecnologia de alimentos

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