Tannase
Mostrando 13-18 de 18 artigos, teses e dissertações.
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13. Produção, purificação, caracterização e aplicação da tanase de Paecilomyces variotii. / Production, purification, characterization and aplication of paecilomyces variotii tannase.
A tanino acil hidrolase conhecida como tanase (E.C: 3.1.1.20) é uma enzima produzida por fungos filamentosos, leveduras e bactérias. São caracterizadas por sua atividade em complexos polifenólicos, sendo capazes de hidrolisar ligações éster (entre o grupo anel aromático e o resíduo de glicose) e ligações depsídicas (ligação éster entre os ané
Publicado em: 2007
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14. Modeling gallic acid production rate by empirical and statistical analysis
Para predizer a taxa das reações enzimaticas uma correlação empírica baseada nos resultados experimentais foi desenvolvida. Os modelos representam a ativação e a desativativação da hydrolise enzimatica. Os resultados foram avaliados pela análise de variança (ANOVA). A atividade máxima da tannase foi obtida após 5 minutos de incubação, temperat
Brazilian Archives of Biology and Technology. Publicado em: 2000
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15. Visual Reading Method for Detection of Bacterial Tannase
Tannase activity of bacteria capable of degrading tannin-protein complexes was determined by a newly developed visual reading method. The method is based on two phenomena: (i) the ability of tannase to hydrolyze methyl gallate to release free gallic acid and (ii) the green to brown coloration of gallic acid after prolonged exposure to oxygen in an alkaline c
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16. Isolation, Purification, and Some Properties of Penicillium chrysogenum Tannase
Tannase isolated from Penicillium chrysogenum was purified 24-fold with 18.5% recovery after ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. Optimum enzyme activity was recorded at pH 5.0 to 6.0 and at 30 to 40°C. The enzyme was stable up to 30°C and within the pH range of 4.0 to 6.5. The Km value wa
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17. Isolation of Tannin-Degrading Lactobacilli from Humans and Fermented Foods
Lactobacilli with tannase activity were isolated from human feces and fermented foods. A PCR-based taxonomic assay revealed that the isolates belong to Lactobacillus plantarum, L. paraplantarum, and L. pentosus. Additional studies on a range of Lactobacillus species from established culture collections confirmed that this enzymatic activity is a phenotypic p
American Society for Microbiology.
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18. Purification and Characterization of Gallic Acid Decarboxylase from Pantoea agglomerans T71
Oxygen-sensitive gallic acid decarboxylase from Pantoea (formerly Enterobacter) agglomerans T71 was purified from a cell extract after stabilization by reducing agents. This enzyme has a molecular mass of approximately 320 kDa and consists of six identical subunits. It is highly specific for gallic acid. Gallic acid decarboxylase is unique among similar deca
American Society for Microbiology.