Reverse Transcriptase Polymerase Chain Reaction
Mostrando 25-36 de 376 artigos, teses e dissertações.
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25. Treatment with 1,25(OH)2D3induced HDAC2 expression and reduced NF-κB p65 expression in a rat model of OVA-induced asthma
Recent evidence indicates that a deficiency of 1,25-dihydroxyvitamin D3 (1,25[OH]2D3) may influence asthma pathogenesis; however, its roles in regulating specific molecular transcription mechanisms remain unclear. We aimed to investigate the effect of 1,25(OH)2D3 on the expression and enzyme activity of histone deacetylase 2 (HDAC2) and its synergistic effec
Braz J Med Biol Res. Publicado em: 28/04/2015
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26. Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
Viruses are the major contributors to the morbidity and mortality of upper and lower acute respiratory infections (ARIs) for all age groups. The aim of this study was to determine the frequencies for a large range of respiratory viruses using a sensitive molecular detection technique in specimens from outpatients of all ages with ARIs. Nasopharyngeal aspirat
Mem. Inst. Oswaldo Cruz. Publicado em: 2014-09
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27. Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alp
Mem. Inst. Oswaldo Cruz. Publicado em: 19/08/2014
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28. The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems’ loc
Mem. Inst. Oswaldo Cruz. Publicado em: 03/07/2014
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29. Inhibitory effect of liposomal quercetin on acute hepatitis and hepatic fibrosis induced by concanavalin A
Immune response plays an important role in the development of hepatic fibrosis. In the present study, we investigated the effects of quercetin on hepatitis and hepatic fibrosis induced by immunological mechanism. In the acute hepatitis model, quercetin (2.5 mg/kg) was injected iv into mice 30 min after concanavalin A (Con A) challenge. Mice were sacrificed 4
Braz J Med Biol Res. Publicado em: 24/06/2014
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30. A comparison of plating and reverse transcriptase polymerase chain reaction followed by microchip electrophoresis for the inactivation of Alicyclobacillus acidoterrestris using saponin
Foi utilizada a reação em cadeia da polimerase via transcriptase reversa (RT-PCR) seguida por eletroforese capilar em microchip para avaliar a viabilidade de esporos de Alicyclobacillus acidoterrestris após a inativação com saponina e temperatura. O método demonstrou ser adequado para tal propósito, além de ser mais rápido e sensível que a tradicio
J. Braz. Chem. Soc.. Publicado em: 2014-01
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31. Expressao genica associada ao estresse oxidativo no coracao de camundongo apos isquemia intestinal
FUNDAMENTO: O fenômeno da isquemia e reperfusão intestinal é um evento frequente na clínica e está associado a repercussões deletérias em órgãos a distância, em especial ao coração. OBJETIVO: Investigar a expressão gênica do estresse oxidativo e defesa antioxidante no coração de camundongos isogênicos, submetidos a isquemia e reperfusã
Arq. Bras. Cardiol.. Publicado em: 14/12/2013
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32. A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy® and a polymerase chain reaction (PCR) assay. Overall, 102 samples from H
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-02
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33. Caracterização genética de rinovírus humano em amostras de populações distintas do Estado de São Paulo. / Human rhinovirus genotyping in samples of distinct populations of São Paulo State, SP, Brazil.
Infections caused by Human Rhinoviruses (HRVs) account for 25%-50% of respiratory illnesses among individuals presenting influenza-like illness (ILI). HRVs could be classified in at least three species: HRV-A, HRV-B, and HRV-C. The HRV-C species has frequently been described among children and apparently has led to severe illness resulting in hospitalization
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2012
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34. Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance
In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes aegypti that were field-caught. Conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and Si
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-11
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35. Infections with human coronaviruses NL63 and OC43 among hospitalised and outpatient individuals in São Paulo, Brazil
The incidence and clinical features of human coronaviruses (HCoVs) among Brazilian patients with respiratory illness are not well known. We investigated the prevalence of HCoVs among Brazilian outpatients and hospitalised patients with respiratory illnesses during 2009 and 2010. To identify the HCoVs, pancoronavirus and species-specific reverse-transcriptase
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-08
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36. Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia
Antiretroviral (ARV) therapy has dramatically reduced morbidity and mortality in human immunodeficiency virus 1 (HIV-1) infected children. However, development of ARV resistance in these children is a major public health problem due to lack of availability of and access to new drugs. This study was conducted in order to identify circulating HIV subtypes and
Brazilian Journal of Infectious Diseases. Publicado em: 2012-06