Proline Glutamate Pathway
Mostrando 1-12 de 32 artigos, teses e dissertações.
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1. Caracterização molecular e bioquímica da prolina desidrogenase de Trypanosoma cruzi, um possível alvo terapêutico. / Molecular and Biochemical Characterization of the proline dehydrogenase of T. cruzi, a possible therapeutical target .
Os nossos resultados demonstraram a atividade enzimática prolina desidrogenase (PRODH) do produto do gene anotado como codificante de uma prolina oxidase na base dados do genoma de Trypanosoma cruzi. A atividade da proteína codificada por esse gene foi avaliada inicialmente por complementação de uma linhagem de S. cerevisiae deficiente na expressão func
Publicado em: 2010
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2. Regulation of Arginine and Proline Catabolism in Bacillus licheniformis
The enzymes in the arginine breakdown pathway (arginase, ornithine-δ-transaminase, and Δ′-pyrroline-5-carboxylate dehydrogenase) were found to be present in Bacillus licheniformis cells during exponential growth on glutamate. These enzymes could be coincidentally induced by arginine or ornithine to a very high level and their synthesis could be repressed
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3. Purification and characteristics of a gamma-glutamyl kinase involved in Escherichia coli proline biosynthesis.
gamma-Glutamyl kinase, the first enzyme of the proline biosynthetic pathway, was purified to homogeneity from an Escherichia coli strain resistant to the proline analog 3,4-dehydroproline. The enzyme had a native molecular weight of 236,000 and was apparently comprised of six identical 40,000-dalton subunits. Enzymatic activity of the protein was detectable
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4. Modulation of Activity of Bacillus subtilis Regulatory Proteins GltC and TnrA by Glutamate Dehydrogenase
The Bacillus subtilis gltAB operon, encoding glutamate synthase, requires a specific positive regulator, GltC, for its expression and is repressed by the global regulatory protein TnrA. The factor that controls TnrA activity, a complex of glutamine synthetase and a feedback inhibitor, such as glutamine, is known, but the signal for modulation of GltC activit
American Society for Microbiology.
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5. Metabolic Changes Associated with Adaptation of Plant Cells to Water Stress 1
Suspension cultured cells of tomato (Lycopersicon esculentum Mill. cv VFNT Cherry) adapted to water stress induced with polyethylene glycol 6000 (PEG), exhibit marked alterations in free amino acid pools (Handa et al. 1983 Plant Physiol 73: 834-843). Using computer simulation models the in vivo rates of synthesis and utilization and compartmentation of free
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6. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: enzyme induction by proline.
Proline is converted to glutamate in the yeast Saccharomyces cerevisiae by the sequential action of two enzymes, proline oxidase and delta 1-pyrroline-5-carboxylate (P5C) dehydrogenase. The levels of these enzymes appear to be controlled by the amount of proline in the cell. The capacity to transport proline is greatest when the cell is grown on poor nitroge
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7. Subcellular compartmentation in control of converging pathways for proline and arginine metabolism in Saccharomyces cerevisiae.
Enzymes of proline biosynthesis and proline degradation which act on the same compound, delta 1-pyrroline-5-carboxylate, are physically separated in yeast cells. The enzyme responsible for the final step in proline biosynthesis, pyrroline-5-carboxylate reductase, converts pyrroline-5-carboxylate to proline and is located in the cytoplasm. The last enzyme in
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8. Proline Excretion and Indirect Suppression in Escherichia coli and Salmonella typhimurium
The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Δ1-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs blocked at an early step in proline biosynthesis (p
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9. Arginine Catabolism in the Cyanobacterium Synechocystis sp. Strain PCC 6803 Involves the Urea Cycle and Arginase Pathway
Cells of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 supplemented with micromolar concentrations of l-[14C]arginine took up, concentrated, and catabolized this amino acid. Metabolism of l-[14C]arginine generated a set of labeled amino acids that included argininosuccinate, citrulline, glutamate, glutamine, ornithine, and proline. Product
American Society for Microbiology.
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10. Amino Acid Metabolism of Lemna minor L. 1: I. Responses to Methionine Sulfoximine
When Lemna minor L. is supplied with the potent inhibitor of glutamine synthetase, methionine sulfoximine, rapid changes in free amino acid levels occur. Glutamine, glutamate, asparagine, aspartate, alanine, and serine levels decline concomitantly with ammonia accumulation. However, not all free amino acid pools deplete in response to this inhibitor. Several
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11. Functional Analysis of the Put3 Transcriptional Activator of the Proline Utilization Pathway in Saccharomyces Cerevisiae
Proline can serve as a nitrogen source for the yeast Saccharomyces cerevisiae when preferred sources of nitrogen are absent from the growth medium. PUT3, the activator of the proline utilization pathway, is required for the transcription of the genes encoding the enzymes that convert proline to glutamate. PUT3 is a 979 amino acid protein that constitutively
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12. Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.
Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of the pathway. Mutants defective in the pro1