In Vitro Enzymatic Digestion
Mostrando 1-12 de 50 artigos, teses e dissertações.
-
1. Inhibitory Effect of Gamma-Secretase on the Survival Rate of Dental Pulp Stem Cells: An in Vitro Study
Abstract Objective: To evaluate in vitro the effect of gamma-secretase inhibition on the survival of dental pulp stem cells. Material and Methods: Sound teeth have been used. Dental pulp stem cells were isolated by enzymatic digestion. An appropriate number of cells were treated with different concentrations of gamma secretase enzyme (DAPT) (1, 3, 6.25, 12
Pesqui. Bras. Odontopediatria Clín. Integr.. Publicado em: 10/10/2019
-
2. Effects of surface proteins and lipids on molecular structure, thermal properties, and enzymatic hydrolysis of rice starch
Abstract Rice starches with different amylose contents were treated with sodium dodecyl sulfate (SDS) to deplete surface proteins and lipids, and the changes in molecular structure, thermal properties, and enzymatic hydrolysis were evaluated. SDS treatment did not significantly change the molecular weight distribution, crystalline structure, short-range orde
Food Sci. Technol. Publicado em: 08/05/2017
-
3. Effects of hydrolysis and digestion in vitro on the activity of bovine plasma hydrolysates as inhibitors of the angiotensin I converting enzyme
The angiotensin I-converting enzyme (ACE) inhibiting activity of bovine plasma hydrolyzates obtained by Alcalase 2.4 L at different degrees of hydrolysis (DH) was evaluated. For the evaluation of ACE inhibition (ACEI), Hippuryl-His-Leu was used as substrate and the amount of hippuric acid liberated by non-inhibiting ACE was determined by spectrophotometry at
Braz. arch. biol. technol.. Publicado em: 31/01/2014
-
4. Antioxidant capacity of amaranth products: effects of thermal and enzymatic treatments
The effect of different process -defatting, protein concentration, thermal treatment, hydrolysis with Alcalase and in vitro digestion- on the antioxidant capacity of amaranth seeds was studied. The antioxidant capacity of the products was determined in methanolic and aqueous extracts and varied from 1.00 to 21.22 and 4.97 to 369.18 µ mol TE/g sample for DPP
Food Sci. Technol. Publicado em: 13/09/2013
-
5. Identificação de peptídeos hipocolesterolemizantes do isolado protéico do grão de amaranto (Amaranthus cruentus L. BRS-Alegria) / Identification of hypocholesterolemic peptides from amaranth seed protein isolate (Amaranthus cruentus L. BRS-Alegria)
Introdução: Dentre os distúrbios relacionados à alimentação, o aumento do colesterol e, conseqüentemente, a incidência de doenças cardiovasculares, representa um importante problema de Saúde Pública. A proteína do amaranto reduz o colesterol plasmático, possivelmente pela presença de peptídeos bioativos, liberados durante sua digestão parcial
Publicado em: 2008
-
6. Bioatividade do grão de amaranto : avaliação in vitro da atividade ligante de acidos biliares e inibidora da enzima conversora de angiotensina. / Bioactivity of the amaranth grain : in vitro assessment of the binding bile acids and inihibitory activity of the angiotensin converting enzyme.
Amaranth has been highlighted as an excellent alternative or complementary source of food protein due to its balanced amino acid composition. The culture of amaranth has been introduced into Brazil on account of its optimum nutritional (high protein content and better biological quality than that of cereal protein) and functional quality, apart from its agri
Publicado em: 2007
-
7. In vitro and in vivo assessment of the antihypertensive activity of comercial hydrolysates from various protein sources. / Avaliação in vitro e in vivo da atividade anti-hipertensiva de hidrolisados comerciais de diversas fontes proteicas.
Angiotensin converting enzyme (ACE) inhibitory peptides present in foods have motivated the interest of many researchers, since there is evidence that the ingestion of these peptides, could aid in the prevention and in the non-medication treatment of hypertension. The anti-hypertensive activity of the peptides has been mainly assessed in relation to their ca
Publicado em: 2006
-
8. "Influência da composição de carreador biodegradável na viabilidade do implante de células mesenquimais indiferenciadas do tecido adiposo humano" / Influence of scaffold composition in the viability of implantation of human adipose derived undifferentiated mesenchymal cells
Human undifferentiated mesenchymal cells were obtained by enzymatic digestion and centrifugation of the product of liposuction. These cells were expanded, in vitro, and implanted subcutaneously in athymic mice. In group I, each animal received the implant of a 0,25cm2 membrane of glycolic acid and trimethylene carbonate, seeded with 1 x 106 of these cells. I
Publicado em: 2004
-
9. Computer-aided nucleic acid secondary structure modeling incorporating enzymatic digestion data.
We present a computer-aided method for determining nucleic acid secondary structure. The method utilizes a program which has the capability to filter matrix diagonal data on the basis of diagonal length, stabilization energy, and chemical and enzymatic data. The program also allows the user to assign selected regions of the structure as uniquely single-stran
-
10. Poly(A) Polymerase Activity in Reovirus
An enzymatic activity which synthesized oligo(A) in vitro was found in highly purified reovirus. The poly(A) polymerase activity was dependent on Mn2+ and utilized only ATP, whereas the virion-associated RNA polymerase required all four ribonucleoside triphosphates and Mg2+. Oligo(A) synthesis was demonstrated with complete virions and infectious subviral pa
-
11. Approaches to sequence analysis of 125I-labeled RNA.
A method is described for the initial steps of sequence analysis of RNase T1-and pancreatic RN-ase-resistant oligonucleotides of RNA containing cytidylate residues labeled in vitro with 125I. In many cases an oligonucleotide sequence can be deduced from a consideration of (i) its relative position in the two-dimensional fingerprint (with DEAE thin layer homo
-
12. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.
Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mR