In Vitro Enzymatic Digestion
Mostrando 13-24 de 50 artigos, teses e dissertações.
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13. Heparin-Like Structures on Respiratory Syncytial Virus Are Involved in Its Infectivity In Vitro
Addition of heparin to the virus culture inhibited syncytial plaque formation due to respiratory syncytial virus (RSV). Moreover, pretreatment of the virus with heparinase or an inhibitor of heparin, protamine, greatly reduced virus infectivity. Two anti-heparan sulfate antibodies stained RSV-infected cells, but not noninfected cells, by immunofluorescence.
American Society for Microbiology.
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14. Analysis of terminal structures of RNA from potato virus X.
The 5'-end structure of potato virus X RNA was determined following enzymatic methylation in vitro. A single 3H-methyl group was introduced into the 2'-position of the 5'-penultimate residue and the end structure was determined as m7GpppG(m)pAp(Xp)3G. This part of the RNA apparently is involved in binding to ribosomes since it can be partially protected agai
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15. Detection of platinum-DNA adducts by 32P-postlabelling.
We developed a sensitive 32P-postlabeling method for the detection of bifunctional intrastrand crosslinks d(Pt-GpG) and d(Pt-ApG) in DNA in vitro and in vivo. After enzymatic digestion of DNA the positively charged platinum adducts were purified from unplatinated products, using strong cation exchange chromatography. Subsequently the samples were deplatinate
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16. A one-step method for in vitro production of tRNA transcripts
Sequencing of a large number of microbial genomes has led to the discovery of new enzymes involved in tRNA biosynthesis and tRNA function. Preparation of a great variety of RNA molecules is, therefore, of major interest for biochemical characterization of these proteins. We describe a fast, cost-effective and efficient method for in vitro production of tRNA
Oxford University Press.
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17. Primary swine intestinal cells as a model for studying Campylobacter jejuni invasiveness.
Swine small-intestinal enterocytes were used to test the invasiveness of Campylobacter jejuni. The cells were removed from the small intestines of 6-h-old piglets by enzymatic digestion. Two clinical C. jejuni isolates invaded swine enterocytes at significantly higher frequencies than an Escherichia coli control strain. The recovered colonies of C. jejuni T1
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18. The influence of chromatin structure on the distribution of DNA repair synthesis studied by nuclease digestion.
The influence of chromatin structure on the distribution of DNA repair synthesis was studied by enzymatic digestion of "repair labeled" nuclei of mouse mammary cells: "repair labeled" nuclei were isolated from pregnancy mammary tissue fragments, treated in vitro with methylmethanesulfonate (MMS) or methylnitrosourea (MNU), and pulse-labeled with 3H-thymidine
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19. Trichosanthin, a potent HIV-1 inhibitor, can cleave supercoiled DNA in vitro.
Trichosanthin, an abortifacient, immunosuppressive and anti-tumor protein purified from the traditional Chinese herb medicine Tian Hua Fen, is a potent inhibitor against HIV-1 replication. Under normal enzymatic digestion conditions, trichosanthin cleaves the supercoiled double-stranded DNA to produce nicked circular and linear DNA. Trichosanthin has no effe
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20. An in vitro system for the editing of ATP synthase subunit 9 mRNA using wheat mitochondrial extracts.
A posttranscriptional modification (C-to-U) at specific positions of plant mitochondrial mRNA leads to changes in the amino acid sequence as well as to the emergence of novel initiation or termination sites. This phenomenon, named RNA editing, has been described for several mitochondrial genes from different plant sources. We have found recently that RNA edi
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21. Metabolic stability of 2' 5'oligo (A) and activity of 2' 5'oligo (A)-dependent endonuclease in extracts of interferon-treated and control HeLa cells.
Extracts of interferon-treated HeLa cells adsorbed to poly(I) . poly(C)-agarose have been used to synthesize 2'5'oligo(A). This oligonucleotide has been characterized by enzymatic digestion with alkaline phosphatase, snake venom phosphodiesterase, T2 ribonuclease and chromatography on DEAE, and PEI-cellulose. The oligonucleotide inhibits protein synthesis in
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22. Mapping of the T-cell recognition sites of Pseudomonas aeruginosa PAK polar pili.
The polar pili of Pseudomonas aeruginosa consist of a subunit protein, pilin, which is a 144-residue polypeptide that contains a hydrophobic N-terminal region and eight hydrophilic regions distributed throughout the remainder of the molecule. T cells from mice immunized with pili or whole bacteria gave good pilus-specific T-cell proliferation responses. To d
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23. The role of surface loops (residues 204-216 and 627-646) in the motor function of the myosin head.
A characteristic feature of all myosins is the presence of two sequences which despite considerable variations in length and composition can be aligned with loops 1 (residues 204-216) and 2 (residues 627-646) in the chicken myosin-head heavy chain sequence. Recently, an intriguing hypothesis has been put forth suggesting that diverse performances of myosin m
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24. Characterization of the DNA-protein complex at the termini of the bacteriophage PRD1 genome.
DNA of bacteriophage PRD1 has protein P8 at its termini. Extracts of infected cells are able to derivatize P8 in vitro with labeled dGTP. Two early proteins, P1 and P8, products of genes I and VIII, respectively, are the only phage proteins necessary for the formation of the protein P8-dGMP complex. This was shown by complementation of extracts from cells in