Highpressure Liquid Chromatography
Mostrando 13-24 de 678 artigos, teses e dissertações.
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13. Aspectos bioquÃmicos e ultraestruturais de Cunninghamella elegans cultivada em meio contendo naftaleno
In the present work the isolate of Cunninghamella elegans (UCP 542), was studied to evaluate the growth behavior in naphthalene medium. The ultrastructure and the ubiquinone systems were also analyzed. The results demonstrated the capacity of the isolate to grow in the presence of the hydrocarbon, however, exhibiting variations in the growth profile. The nap
Publicado em: 2004
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14. Analysis of Plasma Homocysteine Levels in Patients with Unstable Angina
OBJECTIVE - To determine the prevalence of hyperhomocystinemia in patients with acute ischemic syndrome of the unstable angina type. METHODS - We prospectively studied 46 patients (24 females) with unstable angina and 46 control patients (19 males), paired by sex and age, blinded to the laboratory data. Details of diets, smoking habits, medication used, body
Arquivos Brasileiros de Cardiologia. Publicado em: 2002-08
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15. Avaliação da produção de exopolissacarideo insoluvel por duas linhagens de Agrobacterium sp
Some bacteria of Agrobacterium genus isolated from soil samples and not pathogenic produce two extracellular polysaccharides: an acid and soluble succinoglycan and curdlan gum, an insoluble and neutral polymer. The last one, which received the approval of the FDA - Food and Drug Administration - USA - in 1996, is usually used in food industry because of its
Publicado em: 2002
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16. Ochratoxin A: Isolation and Subsequent Purification by High-Pressure Liquid Chromatography
A purification method for ochratoxin A, using liquid-liquid extractions and a final cleanup by high-pressure liquid chromatography, is described.
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17. Isolation of Xanthomegnin from Penicillium viridicatum by Preparative High-Pressure Liquid Chromatography
A method was developed for the production and purification of xanthomegnin from Penicillium viridicatum (NRRL 6430) cultured on rice at 15°C for 29 days. Liquid-liquid extraction followed by high-pressure liquid chromatography afforded 440 mg of crystalline xanthomegnin per kg of rice.
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18. Isolation of Gregatin A from Phialophora gregata by Preparative High-Pressure Liquid Chromatography
A method was developed for the production and purification of gregatin A from Phialophora gregata NRRL 13198 cultured on rice at 20°C for 28 days. Liquid extraction followed by high-pressure liquid chromatography afforded 247.0 mg of crystalline gregatin A per kg of rice.
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19. Comparison of high-pressure liquid chromatography and bioassay for determination of ciprofloxacin in serum and urine.
Ciprofloxacin was given orally to 10 healthy volunteers for seven consecutive doses of 250 mg every 12 h. Serum and urine samples were collected at distinct times between 0 and 96 h and analyzed both by high-pressure liquid chromatography and by a microbiological assay. The detection limits were 0.006 and 0.03 microgram/ml, respectively. For each method, imp
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20. Separation of bacterial ubiquinones by reverse-phase high-pressure liquid chromatography.
A procedure was developed for the separation of ubiquinones by high-pressure liquid chromatography on a reverse-phase C18 column. Ubiquinones Q6 through Q14 were resolved in 20 min and were distinguished from menaquinones by comparing UV spectra at 248 and 275 nm.
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21. High-pressure liquid chromatographic assay of ceftizoxime with an anion-exchange extraction technique.
An anion-exchange extraction method was used in conjunction with high-pressure liquid chromatography for assay of ceftizoxime in 181 serum samples. Comparison of this method with bioassay gave a linear regression line described by Y = 1.11 + 0.98 X, with a correlation coefficient of 0.984. The anion-exchange extraction method is a fast, reliable method of pr
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22. Assay of Gentamicin in Serum by High-Pressure Liquid Chromatography
A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with o-phthaladehyde is used to form fluorescent products for dete
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23. Determination of flumequine and a hydroxy metabolite in biological fluids by high-pressure liquid chromatographic, fluorometric, and microbiological methods.
A sensitive and specific high-pressure liquid chromatographic method is described for the determination of the antibacterial drug flumequine and a major metabolite, 7-hydroxyflumequine, in human plasma and urine. The assay was linear over a concentration range of 1 to 120 micrograms/ml for both compounds. This method is compared with fluorometric and microbi
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24. High-pressure liquid chromatography analysis and single-dose disposition of tobramycin in human volunteers.
A sensitive and specific fluorometric high-pressure liquid chromatography technique was developed to measure both tobramycin and an internal standard (gentamicin C2). The assay utilizes direct extraction of the o-phthalaldehyde derivatives from serum and urine. Coefficients of variation were 7.9% (serum) and 6.0% (urine) at a tobramycin concentration of 1.0