Assay of Gentamicin in Serum by High-Pressure Liquid Chromatography

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RESUMO

A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with o-phthaladehyde is used to form fluorescent products for detection. The possible scope of this method for analysis of other aminoglycoside antibiotics is illustrated by application to sisomicin and netilmicin.

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