Genetic Silencing
Mostrando 1-12 de 152 artigos, teses e dissertações.
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1. A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans
Abstract XRN2 is an evolutionarily conserved 5’-to-3’ exoribonuclease, which degrades or trims various types of RNA in the nucleus. Although XRN-2 is essential for embryogenesis, larval development and reproduction in Caenorhabditis elegans, relevant molecular pathways remain unidentified. Here we create a germline-specific xrn-2 conditional mutant and p
Genetics and Molecular Biology. Publicado em: 2023
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2. Evidence of ectopic recombination and a repeat-induced point (RIP) mutation in the genome of Sclerotinia sclerotiorum, the agent responsible for white mold
Abstract Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transc
Genet. Mol. Biol.. Publicado em: 07/07/2016
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3. Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts
β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediati
Genetics and Molecular Biology. Publicado em: 05/07/2012
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4. Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes.
Genet. Mol. Biol.. Publicado em: 2012
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5. Caracterização de determinantes genéticos envolvidos na qualidade industrial e nutricional do fruto de tomate / Characterization of genetic determinants involved in industrial and nutritional quality of tomato fruit
This thesis addresses tomato fruit metabolism analysis by using three complementary approaches: i) the identification of candidate genes co-located with QTL previously described from metabolic profiles of S. lycopersicum (cv. M82) and S. pennellii introgressed lines, associated with industrial and nutritional quality traits; ii) the structural analyses of th
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/08/2011
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6. Expression analyses of candidate resistance genes in the Rpp4 Asian Soybean Rust resistance locus.
Asian Soybean Rust (ASR), caused Phakopsora pachyrhizi, is considered the most severe soybean disease around the world. Infection of susceptible genotypes leads to early defoliation, incomplete seed development, and yield losses as high as 80%. Five ASR resistance genes have been identified in soybean: Rpp1, Rpp2, Rpp3, Rpp4 and Rpp5. Of particular interest
2010.. Publicado em: 2011
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7. Acúmulo de ricina em sementes de mamona e silenciamento do gene em plantas geneticamente modificadas
Castor bean (Ricinus communis L.) is an oilseed of great economic and social importance. The oil extracted from its seeds is used in biodiesel production and as raw material for chemicals, cosmetics and lubricants. Besides the oil extracted from the seeds, their production chain generates side-products, which can be used as organic fertilizer of good quality
Publicado em: 2010
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8. Biological and molecular characterization of begomoviruses in soybean (Glycine max) and Euphorbia heterophylla, and RNA interference-mediated virus resistance in transgenic soybean plants / Caracterização molecular e biológica de begomovírus de soja (Glycine max) e leiteiro (Euphorbia heterophylla) e resistência a vírus mediada por RNA interferente em plantas transgênicas de soja
Molecular characterization of begomoviruses is of fundamental importance to identify the mechanisms of genetic variability of these important plant pathogens. Genome analysis allows for an understanding of mutation and recombination mechanisms which lead to evolution of new viral species. In Brazil, only a few reports of begomovirus infection of soybean have
Publicado em: 2009
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9. Morfogênese in vitro em tomateiro e berinjela e silenciamento gênico da sintase do mio-inositol-fosfato por RNAi em tomateiro / In vitro morphogenesis in eggplant and tomato plants and silencing of myo-inositolfosfate sintase gene by RNAi in tomato plants
Este trabalho teve como objetivo: i) a otimização das condições de desinfestação de seentes de tomateiros (Solanum lycopersicum Mill.) e berinjela (Solanum melongena L.); ii) a avaliação da influência do tipo de vedação sobre a qualidade das plântulas e explantes oriundos destas; iii) a avaliação do efeito da sonicação sobre a morfogênese in
Publicado em: 2009
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10. Reação à infecção pelo vírus da tristeza dos citros (CTV) em plantas transgênicas de laranja Hamlin(Citrus sinensis (L.) Osbeck) expressando seqüências gênicas do CTV / Reaction to Citrus tristeza virus (CTV) infection of transgenic Hamlinsweet orange (Citrus sinensis (L.) Osbeck) plants transformed with CTV genetic sequences
O vírus da tristeza dos citros (CTV) é uma das maiores ameaças à citricultura mundial. No Brasil, mesmo com a pré-imunização e com a substituição de porta-enxertos, estirpes fortes de CTV ainda causam prejuízos consideráveis. Com o aparecimento da Morte Súbita dos Citros em 1999 e a possível relação desta doença com o CTV, este vírus voltou
Publicado em: 2008
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11. Analysis of the promiscuous gene expression in the thymus of NOD (non obese diabetic) mice during onset of type 1 diabetes mellitus / Análise da expressão gênica promíscua no timo de camundongos NOD (non obese diabetic) durante a emergência do Diabetes melitus tipo 1
Immunologic tolerance is an essential property of the immune system, which controls immune reactions directed against the body self components. The thymus is seen as the main organ involved with the tolerance induction to self antigens, which are expressed by the thymic cells (central tolerance), while the tolerance induction to the diverse other peripheral
Publicado em: 2008
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12. Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
The kinetoplast genetic code deviates from the universal code in that 90% of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp(CCA) is used by both nuclear and mitochondria genes, since all kinetoplast tRNAs are imported into the mitochondria from the cytoplasm. To allow decoding of the mitochondrial UGA
Memórias do Instituto Oswaldo Cruz. Publicado em: 31/07/2007