Enzyme Kinetic
Mostrando 1-12 de 1240 artigos, teses e dissertações.
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1. Expedient Microwave-Assisted Synthesis of Bis(n)-lophine Analogues as Selective Butyrylcholinesterase Inhibitors: Cytotoxicity Evaluation and Molecular Modelling
In the brain of patients with chronic Alzheimer’s disease (AD), the butyrylcholinesterase (BuChE) levels rise while the acetylcholinesterase (AChE) levels decrease. Therefore, development of new selective BuChE inhibitors is of vital importance. Here we present a series of bis(n)-lophine analogues, where two lophine derivatives are connected by a methylene
J. Braz. Chem. Soc.. Publicado em: 2021-06
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2. Characteristics of glucose oxidase immobilized on Magnetic Chitosan Nanoparticles
Abstract Immobilization process is used to facilitate the enzyme recovery and reusability. The aims of this study were to evaluate the effect of different amounts of chitosan on immobilization efficiency of Glucose Oxidase (GOX), pH effect on enzyme activity and changes of kinetic characteristics of enzyme immobilized on Magnetic Chitosan Nanoparticles (MCNP
Food Sci. Technol. Publicado em: 2020-03
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3. On-Flow Ligand Screening Assay Based on Immobilized Nucleoside Diphosphate Kinase B from Homo sapiens
We describe an on-flow zonal affinity-based chromatography assay to screen ligands for the nucleoside diphosphate kinase B enzyme (NME2) from Homo sapiens. For the first time, we have covalently immobilized NME2 on the surface of an open fused silica capillary reactor (NME2-ICER) and placed the reactor before the analytical column, which resulted in a two-di
J. Braz. Chem. Soc.. Publicado em: 24/10/2019
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4. LIPIDS AS COMPETITIVE INHIBITORS OF SUBTILISIN CARLSBERG IN THE ENZYMATIC HYDROLYSIS OF PROTEINS IN RED TILAPIA (Oreochromis sp.) VISCERA: INSIGHTS FROM KINETIC MODELS AND A MOLECULAR DOCKING STUDY
Abstract Protein hydrolysis can improve food’s nutritional, techno-functional and biological properties, which can increase the possibilities of application in industry. The objective of this research article was to study the effect of lipids on the enzymatic kinetics of red tilapia viscera (RTV) hydrolysis with subtilisin Carlsberg. The RTV were hydrolyze
Braz. J. Chem. Eng.. Publicado em: 30/09/2019
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5. Characteristics of glucose oxidase immobilized on Magnetic Chitosan Nanoparticles
Abstract Immobilization process is used to facilitate the enzyme recovery and reusability. The aims of this study were to evaluate the effect of different amounts of chitosan on immobilization efficiency of Glucose Oxidase (GOX), pH effect on enzyme activity and changes of kinetic characteristics of enzyme immobilized on Magnetic Chitosan Nanoparticles (MCNP
Food Sci. Technol. Publicado em: 15/07/2019
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6. Kinetic study of the effect of kiwi fruit actinidin on various proteins of chicken meat
Abstract In this research, the effect of kiwi protein on the physicochemical properties of chicken meat was investigated (Ross breed). Chicken thighs have been marinated in the form of cubes with dimensions of 2 cm in 20 mL of kiwi protein with activity of 0.9 u/mL for 2, 4, 6, 8, 24, 48, 72, and 96 hr, and kept in a refrigerator at 4 ± 1 °C. Then, the enz
Food Sci. Technol. Publicado em: 27/06/2019
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7. Effect of Blanching on Enzyme Activity and Bioactive Compounds of Blackberry
ABSTRACT The inactivation of peroxidase and polyphenol oxidase and the degradation of phenolic compounds, anthocyanins, and antioxidant activity in blackberry were investigated during blanching in water at 80-90 °C, and in steam, from 0 to 10 minutes. The enzyme kinetics indicated the presence of two isoenzymes with different thermal stability, following tw
Braz. arch. biol. technol.. Publicado em: 14/11/2018
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8. Kinetics and Thermodynamics of Thermal Inactivation of β-Galactosidase from Aspergillus oryzae
ABSTRACT For optimization of biochemical processes in food and pharmaceutical industries, the evaluation of enzyme inactivation kinetic models is necessary to allow their adequate use. Kinetic studies of thermal inactivation of β-galactosidase from Aspergillus oryzae were conducted in order to critically evaluate mathematical equations presented in the lite
Braz. arch. biol. technol.. Publicado em: 13/09/2018
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9. Properties of the enzyme acetolactate synthase in herbicide resistant canola
ABSTRACT Mutations in the gene that encodes the enzyme acetolactate synthase (ALS), conferring plant resistance to ALS inhibitor herbicides, can reduce or inhibit enzymatic function. The objective of this work was to determine the functionality and kinetic characteristics of the enzyme ALS and evaluate the action of the herbicides imazapic (imidazolinone) an
Bragantia. Publicado em: 19/07/2018
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10. Genetic engineering of Theileria parva lactate dehydrogenase gene: a new anti-theilerial target
ABSTRACT: Theileria parva is the causative agent of East Coast Fever (ECF), a tick borne disease, which results in major economic losses in cattle. Major problems in dealing with this illness are the high cost of drugs, development of resistance, and absence of effective vaccines. Thus, exploiting new targets for cost effective and higher therapeutic value d
Pesq. Vet. Bras.. Publicado em: 2018-05
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11. BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES
Lactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system,
Quím. Nova. Publicado em: 2017-09
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12. Performance of Chitosan/Glycerol Phosphate Hydrogel as a Support for Lipase Immobilization
A biocompatible and biodegradable hybrid chitosan/glycerol phosphate hydrogel (CS-GP) was prepared and evaluated as support for enzyme immobilization. In this work a lipase sn-1,3 specific from Rhizopus oryzae was physically immobilized in CS-GP by adsorption (PA) and in CS-GP modified chemically with glutaraldehyde (CS-GP-GA) by covalent attachment (CA). Th
Mat. Res.. Publicado em: 29/06/2017