Enzyme Kinetic
Mostrando 13-24 de 1240 artigos, teses e dissertações.
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13. Protease prospecion and determination of its isoenzymes activity in cocoa cultivars (Theobroma cacao L.)
Abstract Our objective was to characterize the protease enzymatic activity and its isoenzymes on cacao cultivars PH 16 and TSH 1188, produced in southern Bahia, linking it to the conditions of the fermentation process. Proteases were extracted and semi-purified, their activities determined changing substrate, pH and temperature, and the values compared with
Food Sci. Technol. Publicado em: 13/04/2017
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14. Purification and characterization of polyphenol oxidase from purslane
Abstract The polyphenol oxidase (PPO) is an enzyme that is responsible for the enzymatic browning of fruits and vegetables. This is generally undesired process and need to be prevented in food technology. PPO from purslane was purified, characterised and the kinetic parameters for three substrates namely, catechol, L-Dopa and 4-methylcatechol were determined
Food Sci. Technol. Publicado em: 20/03/2017
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15. Additives on in vitro ruminal fermentation characteristics of rice straw
ABSTRACT The objective of this study was to evaluate the effects of mineral and protein-energy (MPES), exogenous fibrolytic enzyme supplements (ES), combination of MPES + ES, and straw without supplement (WS) on digestibility, fermentation kinetic parameters, cumulative gas production, methane, CO2 production, and volatile fatty acid concentration of rice st
R. Bras. Zootec.. Publicado em: 2017-03
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16. A broad pH range and processive chitinase from a metagenome library
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene nam
Braz J Med Biol Res. Publicado em: 05/01/2017
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17. Enzyme kinetic modelling and analytical solution of nonlinear rate equation in the transformation of D-methionine into L-methionine in batch reactor using the new homotopy perturbation method
A mathematical model of biotransformation of D-methionine into L-methionine in the cascade of the enzymes such as, D-amino acid oxidase (D-AAO), L-phenylalanine dehydrogenase (L-PheDH) and formate dehydrogenase (FDH) is discussed. The model is based on a system of coupled nonlinear reaction equations under non steady-state conditions for biochemical reaction
Quím. Nova. Publicado em: 2016-12
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18. Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a
Braz. J. Microbiol.. Publicado em: 2016-09
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19. Simultaneous production of amylases and proteases by Bacillus subtilis in brewery wastes
ABSTRACT The simultaneous production of amylase (AA) and protease (PA) activity by Bacillus subtilis UO-01 in brewery wastes was studied by combining the response surface methodology with the kinetic study of the process. The optimum conditions (T = 36.0 °C and pH = 6.8) for high biomass production (0.92 g/L) were similar to the conditions (T = 36.8 °C and
Braz. J. Microbiol.. Publicado em: 2016-09
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20. Thermal inactivation kinetics of partially purified mango pectin methylesterase
Abstract Kinetic parameters of thermal inactivation of pectin methylesterase (PME) in a partially purified mango enzyme extract were determined. The PME of mango partially purified by salting out showed different patterns of thermal inactivation, indicating the presence of a thermostable fraction at 70 °C and a thermolabile fraction at lower temperatures. T
Food Sci. Technol. Publicado em: 30/06/2016
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21. STABILITY IMPROVEMENT OF IMMOBILIZED ALKALINE PHOSPHATASE USING CHITOSAN NANOPARTICLES
Abstract Enzyme engineering via immobilization techniques is a suitable approach for improving enzyme function and stability and is superior to the other chemical or biological methods. In this study chitosan nanoparticles were synthesized using the Ionic Gelation method and were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Elect
Braz. J. Chem. Eng.. Publicado em: 2016-06
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22. EFFECTIVE ALKALINE PEROXIDE OXIDATION PRETREATMENT OF SHEA TREE SAWDUST FOR THE PRODUCTION OF BIOFUELS: KINETICS OF DELIGNIFICATION AND ENZYMATIC CONVERSION TO SUGAR AND SUBSEQUENT PRODUCTION OF ETHANOL BY FERMENTATION USING Saccharomyces cerevisiae
Abstract Shea tree sawdust delignification kinetic data during alkaline peroxide pretreatment were investigated at temperatures of 120 °C, 135 °C, and 150 °C. The activation energy during delignification was 76.4 kJ/mol and the Arrhenius constant was calculated as 8.4 x 106 per min. The reducing sugar yield for the treated to the untreated biomass was abo
Braz. J. Chem. Eng.. Publicado em: 2016-03
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23. Synthesis and Evaluation of Thiazolyl-1H-benzo[
d ]imidazole Inhibitors ofMycobacterium tuberculosis Inosine Monophosphate DehydrogenaseUsing an orthologue-based design approach, we synthesized and assayed a series of thiazolyl-1
H -benzo[d ]imidazole derivatives as inhibitors ofMycobacterium tuberculosis inosine 5'-monophosphate dehydrogenase (Mt IMPDH). From these experiments, a benzo[d ] imidazole compoundJ. Braz. Chem. Soc.. Publicado em: 2015-07
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24. Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum
Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A Plackett-Burman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL afte
Braz. arch. biol. technol.. Publicado em: 2014-12