Bioreactor Cultivation
Mostrando 1-12 de 40 artigos, teses e dissertações.
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1. In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
Abstract This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) w
Crop Breed. Appl. Biotechnol.. Publicado em: 2018-04
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2. Heterotrophic cultivation of Euglena gracilis on chemically pretreated media
Abstract In this research, the impact of chemical agents on the growth of Euglena gracilis and contaminants (S. cerevisiae and B. subtilis) in different cultivation media was studied. E. gracilis was cultivated on modified Hutner and complex medium in Erlenmeyer flasks and a stirred tank bioreactor. H2O2 and antimycin were used as suppressors of contaminant
Braz. J. Chem. Eng.. Publicado em: 2018-01
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3. Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source
Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment a
Braz. J. Microbiol.. Publicado em: 2017-07
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4. Lactobacillus plantarum BL011 cultivation in industrial isolated soybean protein acid residue
Abstract In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aerat
Braz. J. Microbiol.. Publicado em: 2016-12
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5. BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION
Abstract Bioprocess development studies concerning the production of cellulases are of crucial importance due to the significant impact of these enzymes on the economics of biomass conversion into fuels and chemicals. This work evaluates the effects of solid-state fermentation (SSF) operational conditions on cellulase production by a novel strain of Aspergil
Braz. J. Chem. Eng.. Publicado em: 2016-03
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6. Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale
ABSTRACTOxytetracycline (OTC) production byStreptomyces rimosus was studied in batch and fed-batch cultures in shake flask and bioreactor levels using semi-defined medium. First, the effect of glucose concentration on OTC production and growth kinetics was studied intensively. The optimal glucose concentration in the medium was 15 g/L. Higher glucose concent
Braz. arch. biol. technol.. Publicado em: 2015-10
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7. Recombinant Erwinia carotovora l-asparaginase II production in Escherichia coli fed-batch cultures
Asparaginases are the cornerstone therapy of many successful combination regimens for the treatment of acute lymphoblastic leukemia (ALL), the most common malignancy in children and adolescents. The aim of this work was to produce recombinant Erwinia carotovora L-asparaginase II in Escherichia coli fed-batch cultures. Using a robust fed-batch technique with
Braz. J. Chem. Eng.. Publicado em: 2013-06
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8. Cinética do cultivo de Neisseria lactamica em biorreator. / Bioreactor cultivation kinetics of Neisseria lactamica.
Neisseria lactamica está envolvida na aquisição da imunidade natural contra Neisseria meningitidis, causadora da doença meningocócica. Vesículas de membrana externa (OMV) de N. lactamica são antígenos potenciais contra N. meningitidis. Analisou-se a cinética de biomassa, de produção de OMV, da fonte de carbono (lactato), e dos metabólitos, para m
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 31/08/2012
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9. Produção de ácido lático a partir do bagaço da cana de açúcar / Production of lactic acid from sugar cane bagasse
Production of chemical compounds from lignocellulose residues has attracted attention of actual world society. The search for productive alternatives fossil fuels non-dependent, the use of renewable resources and sustainability are the main reasons. In this work, lactic acid production by fermentation of sugars obtained from sugarcane bagasse, essentially gl
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 31/07/2012
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10. Evaluation of different media for the production of cephalosporins by Streptomyces clavuligerus ATCC 27064
The aim of this work was to compare the composition of a complex and soluble culture medium to eight other media described in the literature through the batch cultivation in a conventional bench-scale bioreactor for the production of cephamycin C by a wild strain of Streptomyces clavuligerus. The proposed medium resulted in an antibiotic production 1.5 to 7.
Braz. arch. biol. technol.. Publicado em: 2012-12
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11. Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c
Penicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an incre
Braz. J. Chem. Eng.. Publicado em: 2012-09
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12. Sistema automático de supervisão e controle de cultivos de alta densidade celular de E. coli recombinante
Cultivos de alta densidade celular de E. coli recombinante constituem uma tecnica rapida e economica para producao de proteinas recombinantes. Por meio deste bioprocesso, sao obtidos produtos de alto valor agregado e de grande importancia na industria farmaceutica, tais como insulina, hormonios de crescimento humano e bovino, antigenos proteicos para formula
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/12/2011