Batch Culture
Mostrando 13-24 de 481 artigos, teses e dissertações.
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13. OPTIMIZATION OF THE OPERATING CONDITIONS FOR RHAMNOLIPID PRODUCTION USING SLAUGHTERHOUSE-GENERATED INDUSTRIAL FLOAT AS SUBSTRATE
AbstractBiosurfactants have a wide range of applications in emulsions, separations, and solubilization because these chemicals reduce the surface tension and viscosity of solutions. This work studied rhamnolipid production using a batch bioreactor with a working volume of 1.5 liters, Pseudomonas aeruginosa ATCC (American Type Culture Collection) 10145 strain
Braz. J. Chem. Eng.. Publicado em: 2015-06
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14. Immobilized Biofilm in Thermophilic Biohydrogen Production using Synthetic versus Biological Materials
Biohydrogen production was studied from the vermicelli processing wastewater using synthetic and biological materials as immobilizing substrate employing a mixed culture in a batch reactor operated at the initial pH 6.0 and thermophilic condition (55 ± 1ºC). Maximum cumulative hydrogen production (1,210 mL H2/L wastewater) was observed at 5% (v/v) addition
Braz. arch. biol. technol.. Publicado em: 2015-02
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15. Methane production of two roughage and total mixed ration as influenced by cellulase and xylanase enzyme addition
In recent decades supplementation of animal feeds with exogenous fibrolytic enzymes has substantially improved digestibility and animal performance. However, information related to associated methane production is limited and inconsistent. This study evaluated the effect of cellulase and xylanase enzymes on in vitro methane production of Eragrostis curvula h
Sci. agric. (Piracicaba, Braz.). Publicado em: 2015-02
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16. Screening of wild type Streptomyces isolates able to overproduce clavulanic acid
The selection of new microorganisms able to produce antimicrobial compounds is hoped for to reduce their production costs and the side effects caused by synthetic drugs. Clavulanic acid is a β-lactam antibiotic produced by submerged culture, which is widely used in medicine as a powerful inhibitor of β-lactamases, enzymes produced by bacteria resistant to
Braz. J. Microbiol.. Publicado em: 2014-09
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17. HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
Pichia pastoris is a methylotrophic yeast used as an efficient expression system for heterologous protein production as compared to other expression systems. Considering that every cell must respond to environmental changes to survive and differentiate, determination of endogenous protein related to heat stress responses and hypoxia, it would necessary to es
Braz. J. Microbiol.. Publicado em: 2014-06
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18. Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology
The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation pa
Braz. arch. biol. technol.. Publicado em: 2014-06
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19. Extracellular polysaccharide production by a strain of Pleurotus djamor isolated in the south of Brazil and antitumor activity on Sarcoma 180
Polysaccharides with medicinal properties can be obtained from fruiting bodies, mycelium and culture broth of several fungus species. This work was carried out in batch culture using a stirred tank reactor with two different initial glucose concentrations (40-50 g/L) and pH values (3.0-4.0) to enhance extracellular polysaccharides production by Pleurotus dja
Braz. J. Microbiol.. Publicado em: 04/03/2014
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20. A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol
This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respect
Braz. arch. biol. technol.. Publicado em: 2013-10
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21. Laboratory apparatus to evaluate microalgae production
The application of microalgae for energy purposes and CO2 biomitigation continues to present a number of challenges, including the optimization of culture conditions. The application of experimental designs for microalgae cultivation is difficult, since experiments involving such microorganisms generally last days or weeks. This work proposes a multipurpose
Braz. J. Chem. Eng.. Publicado em: 2013-09
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22. Recombinant Erwinia carotovora l-asparaginase II production in Escherichia coli fed-batch cultures
Asparaginases are the cornerstone therapy of many successful combination regimens for the treatment of acute lymphoblastic leukemia (ALL), the most common malignancy in children and adolescents. The aim of this work was to produce recombinant Erwinia carotovora L-asparaginase II in Escherichia coli fed-batch cultures. Using a robust fed-batch technique with
Braz. J. Chem. Eng.. Publicado em: 2013-06
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23. Hexavalent chromium reduction by immobilized cells of Bacillus sphaericus AND 303
Bacillus sphaericus AND 303, a Cr(VI)-resistant and reducing bacterium reported from serpentine outcrops of Andaman was evaluated for Cr(VI) reduction using immobilized cells under batch culture. Screening of inert matrices for entrapment of whole cells indicated that polyvinyl alchohol-alginate was the most effective one reducing 87.5% of 20 µM Cr(VI) in 2
Braz. arch. biol. technol.. Publicado em: 2013-06
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24. Semi-scale production of PHAs from waste frying oil by Pseudomonas fluorescens S48
The present study aimed at developing a strategy to improve the volumetric production of PHAs by Pseudomonas fluorescens S48 using waste frying oil (WFO) as the sole carbon source. For this purpose, several cultivations were set up to steadily improve nutrients supply to attain high cell density and high biopolymer productivity. The production of PHAs was ex
Braz. J. Microbiol.. Publicado em: 2013