Visualization of plant cell walls by atomic force microscopy.

AUTOR(ES)

Kirby, A R

RESUMO

Atomic force microscopy has been used to visualize the ultrastructure of hydrated plant cell wall material from prepared apple (Malus pumila MILL; Cox orange pippin), water chestnut (Eleocharis dulcis L.), potato (Solanum tuberosum L.; Bintje), and carrot (Daucus carota L.; Amsterdamse bak) parenchyma. Samples of cell wall material in aqueous suspension were deposited onto freshly cleaved mica. Excess water was blotted away and the moist samples were imaged in air at ambient temperature and humidity. The three-dimensional images obtained highlighted the layered structure of the plant cell walls and revealed features interpreted as individual cellulose microfibrils and plasmodesmata.

Documentos Relacionados

• Visualization of RNA crystal growth by atomic force microscopy.
• Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy.
• Molecular resolution imaging of macromolecular crystals by atomic force microscopy.
• High resolution mapping DNAs by R-loop atomic force microscopy.
• Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy.