Direct visualization of polypeptide shell of ferritin molecule by atomic force microscopy.
AUTOR(ES)
Ohnishi, S
RESUMO
The polypeptide shell of the ferritin molecule has been imaged in water by atomic force microscopy (AFM). The central dip and the quaternary structure could be observed on the surface of the ferritin molecule anchored inhomogeneously in two dimensions. These structures observed in the AFM images are quite similar to the electron density map near the top of the apoferritin viewed down from a 4-fold axis structure reported previously (S. H. Banyard, D. K. Stammers, and P. M. Harrison, 1978. Nature (Lond.). 271:282-284). It has been achieved by introducing a "self-screening effect" of the surface charges of the AFM sample (S. Ohnishi, M. Hara, T. Furuno, and H. Sasabe. 1992. Biophys. J. 63:1425-1431) and the specially sharpened stylus of AFM cantilever.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1225760Documentos Relacionados
- Visualization of RNA crystal growth by atomic force microscopy.
- Visualization of plant cell walls by atomic force microscopy.
- Direct observation of protein secondary structure in gas vesicles by atomic force microscopy.
- Direct measurement of hydrogen bonding in DNA nucleotide bases by atomic force microscopy.
- Molecular resolution imaging of macromolecular crystals by atomic force microscopy.