TÉCNICAS AVANÇADAS PARA A DIFERENCIAÇÃO DE ISÔMEROS POR ESPECTROMETRIA DE MASSAS
AUTOR(ES)
Penna, Tatiana C.; Correra, Thiago C.
FONTE
Quím. Nova
DATA DE PUBLICAÇÃO
2020-09
RESUMO
Mass spectrometry (MS) is a powerful and widespread technique but is inherently uncapable to differentiate species with same m/z ratio. For this reason, many classical approaches were developed based on differential fragmentation patterns in tandem MS. More recently, new techniques for isomer identification in MS were developed, and we highlight the photodissociation techniques that will be discussed in this review. This photodissociation techniques explore the selective activation of isomers to induce isomer specific dissociations in the gas-phase that depend on the spectroscopic features of the isomers. If tunable radiation sources are available, this selective activation may be monitored as a function of the radiation wavelength, allowing the acquisition of vibrational or UV-Vis spectra of the isolated ions, making it possible to evaluate different isomers, protomers and even conformers by MS. These photodissociation techniques are even more powerful when coupled to ion mobility techniques, that will be briefly discussed. Because these techniques enhance the fields and analytical applications where MS can be used, this review will discuss the classical MS methods for isomer differentiation that lead to the fundamentals of ion spectroscopy and selective ion activation, with emphasis on the vibrational ion spectroscopy.
Documentos Relacionados
- Tecnicas avançadas em espectrometria de massas - MIMS e suas derivadas CT-MIMS
- Atribuição absoluta e geral de isômeros constitucionais por espectrometria de massas: o caso das metilpiperidinas
- Desenvolvimento de software e hardware para espectrometria de massas e cromatografia gasosa/espectrometria de massas
- Principais técnicas de preparo de amostra para a determinação de resíduos de agrotóxicos em água por cromatografia líquida com detecção por arranjo de diodos e por espectrometria de massas
- Cromatografia gasosa-espectrometria de massas e derivatização assistida por micro-ondas na identificação de isômeros de glicose: uma prática para o ensino avançado em análise e caracterização de compostos orgânicos