Solid-phase immune electron microscopy with human immunoglobulin M for serotyping of Norwalk-like viruses.
AUTOR(ES)
Lewis, D C
RESUMO
A solid-phase immune electron microscopy method that uses protein A, goat anti-human immunoglobulin M (IgM), and human serum is described. Evaluation of the method with different immunoglobulin fractions showed that human IgM constituted the major virus capture antibody. The method appeared to distinguish between two Norwalk-like virus serotypes and demonstrated specific IgM responses to these serotypes in infected individuals. Further work is being carried out to define the relationship of these two serotypes to the previously described Norwalk agent (A. Z. Kapikian, R. G. Wyatt, R. Dolin, T. S. Thornhill, A. R. Kalica, and R. M. Chanock, J. Virol. 10:1075-1081, 1972), and four subsequent hospital outbreaks are being studied.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266490Documentos Relacionados
- Use of solid-phase immune electron microscopy for classification of Norwalk-like viruses into six antigenic groups from 10 outbreaks of gastroenteritis in the United States.
- Rapid serotyping of human rotavirus strains by solid-phase immune electron microscopy.
- Comparative sensitivities of solid-phase immune electron microscopy and enzyme-linked immunosorbent assay for serotyping of human rotavirus strains with neutralizing monoclonal antibodies.
- Solid-phase immune electron microscopy-double-antibody technique for rapid detection of papovaviruses.
- Immunoglobulin M Antibody Test To Detect Genogroup II Norwalk-Like Virus Infection