Solid-phase immune electron microscopy-double-antibody technique for rapid detection of papovaviruses.
AUTOR(ES)
Giraldo, G
RESUMO
The solid-phase immune electron microscopy-double-antibody technique, which takes less than 1 h to perform, was applied as a rapid, sensitive, and specific diagnostic tool in the demonstration of papovavirus particles. BK virus propagated in 82C human skin fibroblasts and a monospecific high-titer immune serum to BK virus were used to establish the test procedure. When Formvar-carbon-coated grids were treated with appropriately diluted antibody, a 28-fold increase of virus particles per square micrometer was observed. Viewing of the virus particles was facilitated by the addition of a second "decorator" antibody. BK virus preparations at concentrations of 10(2) to 10(3) PFU/ml could be detected by this technique. There was no cross-reaction with mouse polyomavirus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272128Documentos Relacionados
- Rapid serotyping of human rotavirus strains by solid-phase immune electron microscopy.
- Sensitive solid-phase immune electron microscopy double-antibody technique with gold-immunoglobulin G complexes for detecting rotavirus in cell culture and feces.
- Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.
- Solid-phase immune electron microscopy with human immunoglobulin M for serotyping of Norwalk-like viruses.
- Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens.