Simple assay of calcium dependency for virulent plasmid-bearing clones of Yersinia enterocolitica.

AUTOR(ES)

Bhaduri, S

RESUMO

A simplified procedure to detect the calcium dependency of virulent plasmid-bearing strains of Yersinia enterocolitica was developed. A low-calcium, agarose-based medium of brain heat infusion with added magnesium effectively differentiated plasmid-bearing and plasmidless isolates. Further, the expression of calcium dependency in plasmid-bearing strains of Y. enterocolitica as measured by the average colony diameter was proportional to the calcium concentration of the assay.

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