Assay of crystal violet binding for rapid identification of virulent plasmid-bearing clones of Yersinia enterocolitica.

AUTOR(ES)

Bhaduri, S

RESUMO

A rapid, reliable, and simple method based on the binding of crystal violet (CV) is described for differentiating virulence-plasmid-bearing strains of Yersinia enterocolitica from their plasmidless derivatives. As with other plasmid-mediated properties of this organism, the binding of CV occurs at 37 degrees C but not at 25 degrees C. The CV-binding technique provides a simple and efficient means of screening Y. enterocolitica for virulence and for identifying individual plasmid-bearing colonies.

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