Quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus

AUTOR(ES)

Silva, Leonardo C., Machado, Rúbia D., Silva, Dayane R., Amaral, Vanessa C.S., Paula, José R., Conceição, Edemilson C., Paula, Joelma A.M.

FONTE

Rev. bras. farmacogn.

DATA DE PUBLICAÇÃO

2017-10

RESUMO

Abstract This paper describes the quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus (Gomes) Landrum, Myrtaceae, citral chemotype using a validated HPLC-PDA method. The method employs a RP-18 column with acetonitrile:water-orthophosphoric acid 0.05% (gradient system) and UV detection at 210 nm. The method was demonstrated to be simple, sensitive, specific, linear, precise, accurate and robust. The response was linear over a range of 5–200 µg/ml (r > 0.999). The range of recoveries was 92.27–102.54%. The relative standard deviation values for intra- and inter-day precision studies were 4.30 and 3.78%, respectively. This assay can be readily utilized as quality control method for catechin in the dried extract of P. pseudocaryophyllus.

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