[PROVISIONAL] Identification of histone methylation modifiers and their expression patterns during somatic embryogenesis in Hevea brasiliensis

AUTOR(ES)
FONTE

Genet. Mol. Biol.

DATA DE PUBLICAÇÃO

22/08/2019

RESUMO

Abstract Histone methylation plays a crucial role in various biological processes including from heterochromatin formation to transcriptional regulation. Currently, no information is available regarding histone methylation modifiers in the important rubber-producing plant Hevea brasiliensis. Here, we identified 47 histone methyltransferase (HMT) genes and 25 histone demethylase (HDM) genes, as possible members of the histone methylation modifiers in the rubber tree genome. According to the structural features of HMT and HDM, the HbHMTs were classified to two groups (HbPRMs and HbSDGs), the HbHDMs have two groups (HbLSDs and HbJMJs). The expression patterns of HbHMTs and HbHDMs were analyzed in five different tissues and at different phases of somatic embryogenesis. HbSDG10, 21, 25, 33, HbJMJ2, 18, 20 were with high expression at different phases of somatic embryogenesis. HbSDG10,14, 20, 21, 3 and HbPRMT4 were expressed highly in anther, HbSDG14, 20, 21, 22, 23, 33, 35 and HbPRMT1 HbJMJ7 and HbLSD1, 2, 3, 4 showed a high expression level in callus. HbSDG1, 7, 10, 13, 14, 18, 19, 21, 22, 23, 35, HbPRMT1, 8, HbJMJ5, 7, 11, 16, 20 and HbLSD2, 3, 4 were expressed highly in somatic embryo. HbSDG10, 21, 25, 33, HbLSD2, 3 3 were expressed highly in bud of regenerated plant. The expression analyses reveal that HbHMTs and HbHDMs exhibit different expression patterns at different phases during somatic embryogenesis and imply that some HbHMTs and HbHDMs play important roles during somatic embryogenesis. This study will provide fundamental to further study on regulations of histone metylation in Hevea brasiliensis.

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