Coordinate gene expression during somatic embryogenesis in carrots

AUTOR(ES)

Sung, Z. R.

RESUMO

There are several biochemical differences between the callus and the embryos of carrot culture. Callus tissue produces callus-specific proteins and a conditioning factor that is necessary for the synthesis of callus-specific proteins. By contrast, embryos produce embryo-specific proteins [Sung, Z. R. & Okimoto, R. (1981) Proc. Natl. Acad. Sci. USA 78, 3683-3687] and develop the capability to inactivate cycloheximide [Sung, Z. R., Lazar, G. J. & Dudits, D. (1981) Plant Physiol. 68, 261-264]. A mutant, WCH105, that can inactivate cycloheximide in the callus as well as in the embryos produces the embryo-specific proteins instead of the callus-specific proteins and fails to produce the conditioning factor by the callus tissue. Callus tissues also produce a conditioning factor for callus growth. This factor is not the same as the conditioning factor for the synthesis of the callus-specific proteins, as WCH105 can grow as callus. The existence of WCH105 demonstrates that the callus-specific and embryo-specific traits are coordinately regulated, but in an opposite manner. A common mechanism apparently activates one set and inactivates the other set of functions. WCH105 seems to be impaired in this mechanism.

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