Method validation for determination of metals in Vitis labrusca L. grapevine leaf extracts by inductively coupled plasma mass spectrometry (ICP-MS)

AUTOR(ES)

BOKOWSKI, LIANE V.V., SOBRINHO, ROBERTO B., ARMIJO, CRISTIÁN J.V., DANI, CAROLINE, HENRIQUES, JOÃO A.P., FUNCHAL, CLÁUDIA

FONTE

An. Acad. Bras. Ciênc.

DATA DE PUBLICAÇÃO

2016-12

RESUMO

ABSTRACT Vitis labrusca L. is the main species used for wine and juice production in Brazil. The grapevine leaves can be used both as functional foods and as cheapest sources for the extraction of phenolic compounds. Besides the antioxidant activity, grapevine leaves exhibited significant anti-inflammatory activity. Therefore, the aim of this study was to develop and validate an analytical methodology to determine the metals selenium (96Se), chromium (53Cr), nickel (62Ni), cadmium (111Cd) and lead (206Pb) in 30 samples of grapevine leaf extracts (Vitis labrusca, Bordo cultivar) using inductively coupled plasma mass spectrometry (ICP-MS). To obtain the grapevine leaf extracts the samples were milled, weighed and digested in microwave oven with nitric acid. The method showed linearity, precision, accuracy and limits of quantification and detection acceptable for INMETRO protocol validation of analytical methods. Therefore, the method using ICP-MS was developed and validated to determine metals concentrations in grapevine leaves of Vitis labrusca L. and the proposed method could be applied in routine analytical laboratory.

Documentos Relacionados

• Determination of Boron Isotope Ratios in Tooth Enamel by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) After Matrix Separation by Ion Exchange Chromatography
• Fast method of multi-elemental analysis of stream sediment samples by inductively coupled plasma-mass spectrometry (ICP-MS) with prior single-step microwave-assisted digestion
• Pure cerium dioxide preparation for use as spectrochemical standard and analysed by inductively coupled plasma mass spectrometry (SF ICP-MS)
• Validation method to determine metals in atmospheric particulate matter by inductively coupled plasma optical emission spectrometry
• Evaluation techniques coupled to mass spectrometry (ICP-MS) aimed at the fractionation and chemical speciation of mercury in blood and plasma