Evaluation techniques coupled to mass spectrometry (ICP-MS) aimed at the fractionation and chemical speciation of mercury in blood and plasma / Avaliação de técnicas acopladas à espectrometria de massas com plasma (ICP-MS) visando o fracionamento e a especiação química de mercúrio em sangue e plasma

AUTOR(ES)

Jairo Lisboa Rodrigues

DATA DE PUBLICAÇÃO

2010

RESUMO

Mercury (Hg) is one of the most toxic environmental pollutants. It exists primarily in three forms: elemental mercury (Hg0), or metallic mercury, inorganic mercury (Hg-i), particularly mercuric chloride and organic mercury (Hg-o), mainly represented by methylmercury (MeHg) and ethylmercury (EtHg), and the organic forms of mercury are more toxic than the inorganic ones. Then, it is very important the development of simple and fast methods for mercury fractionation (T-Hg, Hg-i and Hg-o by the difference) or speciation (Hg-i, MeHg, EtHg) in biological samples. Then, the aims of this work were to evaluate three analytical methods for: i) mercury fractionation in blood/plasma samples (Hg-t, Hg-i and Hg-o by difference) by using a ICP-MS on line coupled to a cold-vapor generation system (CV ICP-MS), ii) Hg speciation in blood and plasma by using LC coupled to ICP-MS; iii) Hg speciation in blood samples with the use of GC coupled to ICP-MS. For the fractionation method, samples were previously incubated with tetramethylammonium hydroxide (TMAH) at room temperature. On the other hand, for the speciation of Hg in blood/plasma by using HPLC-ICP-MS the extraction of Hg species was carried out with the use of ultrasonic energy. For the speciation methodology with GC-ICP-MS the extraction of Hg species was carried out with the use of microwave-assisted extraction. Validation of the proposed methods were evaluated based on the analysis of the SRM NIST 966 and ordinary blood samples collected from riparians living in the Brazilian Amazon exposed to mercury. In general the proposed methodologies proved to be simple, fast and easily applied in routine analysis by clinical laboratories.

ASSUNTO(S)

hplc-icp-ms sangue blood mercury gc-icp-ms vapor frio mercúrio cold vapor speciation hplc-icp-ms gc-icp-ms

Documentos Relacionados

• Method validation for determination of metals in Vitis labrusca L. grapevine leaf extracts by inductively coupled plasma mass spectrometry (ICP-MS)
• Determination of Boron Isotope Ratios in Tooth Enamel by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) After Matrix Separation by Ion Exchange Chromatography
• Desenvolvimento de método para determinação de Ag, As, Cd, Co, Mn, Ni, Pb e Se em sangue por espectrometria de massas com fonte de plasma acoplado indutivamente (ICP-MS) utilizando diluição das amostras em meio alcalino
• Fast method of multi-elemental analysis of stream sediment samples by inductively coupled plasma-mass spectrometry (ICP-MS) with prior single-step microwave-assisted digestion
• Pure cerium dioxide preparation for use as spectrochemical standard and analysed by inductively coupled plasma mass spectrometry (SF ICP-MS)