Mapeamento comparativo do cromossomo 14 bubalino (Bubalus bubalis), em painel de células somáticas híbridas irradiadas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The development of genomic tools and the generation of molecular data for the river buffalo (Bubalus bubalis) are still in an initial phase in relation to other species of interest to animal production activities. In this context, this work will be of great importance for studies geared to identifying the genetic basis and molecular mechanisms that result in significant differences between buffaloes and cattle, both in production physiology and meat and milk quality and composition. The last generation genomic tool for constructing physical maps is the Radiation Hybrid Panel (RH Panel), because it allows the use of markers derived from related species and useful for comparative mapping. Recently, an international consortium was established for the construction of a first generation buffalo RH map, and the present study was carried out as a contribution to the International Consortium. PCR amplification conditions were tested for 49 markers derived from bovine gene sequences located on chromosome 13 (BTA13), which has a high degree of sinteny with buffalo chromosome 14 (BBU14). Ideal amplification conditions were obtained for a total of 37 markers, which were tested at least twice in all RH cell linesto minimise the occurrence of false positive or negative scorings. The program Carthagene was used to analyze the data and generate the RH map. The result obtained showed high syntenic conservation between chromosomes BTA13 and BBU14, with some discrepancies, which were not supported by final map generated by the consortium. This study was important for the construction of the first generation RH genome map of river buffalo.

ASSUNTO(S)

rh map búfalo de rio genômica ciencias agrarias genomics chromossome mapping mapeamento cromossômico mapa rh river buffalo

ACESSO AO ARTIGO

Documentos Relacionados