Ion-Diffusion Potentials and Electrical Rectification Across Lipid Membranes Activated by Excitation-Induced Material

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RESUMO

Membranes synthesized from sphingomyelin-tocopherol mixtures and treated with the protein, “excitation-inducing material,” to increase their electrical conductance were tested for applicability of the Nernst equation and changes in electrical rectifying behavior with temperature. Above pH 7, and in the temperature range 303-323°K, ion-diffusion potentials created by salts of monovalent cations showed that areas of the membrane activated by excitation-inducing material were only permeable to cations. Results also indicated that a parallel current with an activation energy of 1.00 eV per molecule passed through unactivated areas of the membrane. Below pH 7, the ratio of measured diffusion potentials to predicted values decreased steadily.

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