Influência das proteínas salivares e plasmáticas no desenvolvimento de biofilmes de Candida albicans / The influence of salivary and plasmatic proteins on the development of Candida albicans biofilms

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

15/02/2012

RESUMO

The development of Candida albicans biofilms may be mediated by a differential expression of proteic ligand sites in the acquired pellicle formed onto the oral prosthesis surfaces. Therefore, the main objective of this study was to evaluate the influence of salivary and plasmatic-derived proteins on the development of C. albicans biofilms. In the first chapter the state-of-the-art was reviewed for methodologies applied to the analysis of proteins. From the knowledge of the methodologies, the research was realized aiming to characterize the protein profile of acquired pellicles (ADP) on poly(methylmetacrilate) surfaces specimens in the presence of saliva or saliva supplemented with blood plasma. ADP composition was analyzed by mass spectrometry techniques, which results demonstrated a significant difference in the obtained proteome between the groups. Moreover, the influence of these pellicles was evaluated for the surface free energy (SFE) and expression of virulence factors by C. albicans biofilms. The findings demonstrated that the binding of plasmatic proteins in the ADP determined increases in both experimental variables. From these data, the individual effect of some identified ADP proteins was evaluated on C. albicans biofilm development. With this objective, C. albicans biofilms were developed (90 min, 24, 48 and 72 h) over mono-protein pellicles of albumin; mucin I and II; lactoferrin; fibrinogen; C3b; IgA and histatin 5. For each time point, biofilms were analyzed for metabolic activity (XTT assay), structural biofilm conformation (Confocal scanning laser microscopy) and, expression of virulence factors by means of enzymatic activity assays. Based on the generated evidences of these studies, we can conclude that adsorbed salivary and plasmatic proteins as proteic integuments keep their biological function, and thus exert a modulatory influence on C. albicans biofilm development.

ASSUNTO(S)

saliva virulencia (microbiologia) espectrometria de massa candida albicans saliva microbial virulence mass spectrometry candida albicans

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