Influência da Proteína Morfogenética Óssea-7 (BMP-7) sobre o desenvolvimento in vitro de folículos pré-antrais caprinos. / Influence of bone morphogenetic protein-7 (BMP-7) on the in vitro development of caprine preantral follicles.

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

11/12/2008

RESUMO

The aim of this study was to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (0, 1, 10, 50 or 100 ng/ml). Noncultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. After 1 or 7 days of culture, the percentage of morphologically normal follicles significantly reduced in all treatments when compared with fresh control, except at 1 ng/ml of BMP-7 for 1 day. In addition, the concentration of 10 ng/ml of BMP-7 significantly increased follicular diameter from day 1 to 7 of culture. There was not any influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1 ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival of caprine preantral follicles during in vitro culture.

ASSUNTO(S)

caprinos folículos pré-antrais cultivo sobrevivência bmp-7 reproducao animal caprine preantral follicles culture survival bmp-7

ACESSO AO ARTIGO

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