Preantral Follicles
Mostrando 1-12 de 53 artigos, teses e dissertações.
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1. Growth and Differentiation Factor-9 Supplementation Affects Viability and Morphology of Preantral Follicles in Equine Ovarian Fragments During Short-term in vitro Culture
Abstract This study aimed to evaluate different concentrations of growth and differentiation factor-9 (GDF-9) on the development and maintenance of equine preantral follicle morphology during short-term in vitro culture. Ovaries (n=5) from five mares were collected from a local slaughterhouse and transported to the laboratory, where nine fragments (5x5x1mm)
Braz. arch. biol. technol.. Publicado em: 20/12/2019
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2. Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue
Abstract This study evaluated a powdered coconut water solution (ACP 406®) as a base culture medium on the in vitro survival and development of in situ goat preantral follicles. The ovarian fragments were either immediately fixed in Carnoy solution (non-cultured control) or individually cultured for 2 or 6 days. The following culture media (all containing 1
Anim. Reprod.. Publicado em: 25/11/2019
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3. Morfometria e população de folículos pré-antrais de vacas Nelore vazias, gestantes e de fetos nos terços inicial, médio e final da gestação
ABSTRACT The aim of this study was to describe population and morphological characteristics of preantral follicles of not pregnant cows, pregnant cows and fetus. Ten ovaries of non-pregnant Nelore cows, eighteen ovaries of pregnant cows and eighteen ovaries of fetus were used. For pregnant cows, six ovaries from each third (initial, middle and final) were ev
Arq. Bras. Med. Vet. Zootec.. Publicado em: 28/10/2019
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4. Effect of somatotropin on survival and diameter of bovine preantral follicles
RESUMO O objetivo deste trabalho foi avaliar o efeito da somatotropina recombinante bovina (rbST) sobre a sobrevivência e o diâmetro de folículos ovarianos pré-antrais (FOPA) bovinos cultivados in vitro. Ovários foram coletados de vacas adultas e fragmentos do córtex ovariano foram imediatamente fixados (controle não cultivado) ou cultivados in vitro
Arq. Bras. Med. Vet. Zootec.. Publicado em: 28/10/2019
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5. DNA damage and primordial follicle activation after in vitro culture of sheep ovarian cortex in Morus nigra leaf extract
RESUMO: Este estudo avaliou o efeito do extrato das folhas de Morus nigra, com ou sem suplementos, sobre a morfologia, a ativação e o dano ao DNA de folículos pré-antrais cultivados inclusos em tecido ovariano. Os ovários foram coletados e divididos em fragmentos, sendo um fixado para análise histológica e ensaio de marcação de terminações dUTP me
Pesq. Vet. Bras.. Publicado em: 2019-01
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6. Anethole reduces oxidative stress and improves in vitro survival and activation of primordial follicles
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concent
Braz J Med Biol Res. Publicado em: 28/05/2018
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7. Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles
The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At
Braz. arch. biol. technol.. Publicado em: 2014-04
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8. Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). Aft
Braz J Med Biol Res. Publicado em: 19/03/2013
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9. EFEITO DA ASSOCIAÇÃO DO FATOR DE CRESCIMENTO E DIFERENCIAÇÃO 9 (GDF-9) E DO HORMÔNIO FOLÍCULO ESTIMULANTE (FSH) SOBRE O DESENVOLVIMENTO IN VITRO DE FOLÍCULOS PRÉ-ANTRAIS CAPRINOS EM CULTIVO IN SITU DE LONGA DURAÇÃO / EFFECT OF ASSOCIATION AND GROWTH FACTOR DIFFERENTIATION 9 (GDF-9) and hormone FOLLICLE STIMULATING (FSH) DEVELOPMENT OF IN VITRO PREANTRAL FOLLICLES GOATS IN FARMING IN SITU LONG
The aim of this study was to evaluate the effects of growth differentiation factor 9 (GDF-9) and follicle-stimulating hormone (FSH) on the in vitro survival, growth and steroidogenesis of caprine preantral follicles cultured for 16 days. Ovarian fragments were cultured in αMEM+ in the presence or absence of GDF-9 (200 ng/mL) and/or FSH (50 ng/mL) added
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/07/2012
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10. Cultivo de folÃculos prÃ-antrais caprinos em meio suplementado com a lectina PHA de Phaseolus vulgaris. / Cultivation of goat preantral follicles in medium supplemented with the lectin PHA from Phaseolus vulgaris.
Os caprinos sÃo considerados importantes fontes de carne, leite e pele, e, portanto, desempenham importante papel econÃmico especialmente na regiÃo nordeste do Brasil Diante disso, o desenvolvimento de sistemas de cultivo de folÃculos ovarianos pode otimizar potencial reprodutivo desta espÃcie. O objetivo deste trabalho foi avaliar o efeito da lectin PH
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/03/2012
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11. Análise do mRNA da proteína BMP-15 e IGF-2 em oócitos e células do cumulus de ovelhas do semiárido pernambucano. / Analysis of mRNA expression of BMP-15 and IGF-2 proteins in oocytes and cumulus cells of sheep in semi-arid state of Pernambuco.
Two experiments were conducted to analyze the expression of genes related to follicular development and subsequent reproductive efficiency in oocytes and cumulus cells in two distinct phases of folliculogenesis. Ovaries of native breeds of sheep slaughtered in the abattoir in Caruaru/PE were collected and transported to the Laboratory of Applied Molecular An
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/02/2012
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12. Efeitos do fator de crescimento e diferenciaÃÃo-9 e do hormÃnio folÃculo estimulante sobre o desenvolvimento in vitro de folÃculos prÃ-antrais bovinos. / Follicle isolated analyzed by fluorescence microscope (A, B) Preantral follicle cultured for 12 days in MEM (C, D) Preantral follicle cultured for 12 days in GDF-9 (E, F) Follicle preantral cultured for 12 days in GDF + FSH (G, H) Preantral follicles cultured for 12 days in FSH. Scale bar represents 50 micrometers
O objetivo deste estudo foi determinar o papel do GDF-9 sozinho ou em combinaÃÃo com FSH sobre a viabilidade, crescimento e expressÃo do RNAm para PCNA, HAS 1, HAS 2, versican e perlecan em folÃculos secundÃrios bovinos cultivados in vitro. Para estudos in vitro, folÃculos secundÃrios bovinos foram isolados e cultivados por doze dias, na presenÃa de
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2012