Indução de mutantes auxotroficos de Xanthomonas campestris (Pam) Dowson e estudo de sua patogenicidade em plantulas de repolho (Brassica oleracea var. capitata L.)

AUTOR(ES)

Fausto Foresti

DATA DE PUBLICAÇÃO

1972

RESUMO

Auxotrophic mutants of Xanthomonas campestris were induced by ultraviolet irradiation, followed by penicillin treatment. The mutant yield was approximately one mutant per 1,4 x 10 POT. 2 tested colonies. The nutritional requirements of these mutants were determined auxonographically and were specific in that no other growth factor would support growth. Twelve amino acid requiring mutants were obtained. Three of these mutants were tested for their pathogenicity on cabbage plants in laboratory and green-house experiments. The auxotrophic mutations seemed to have effects on pathogenicity. Mutants showed to be avirulent in vitro even whem their required substances were furnished from a external source to the host. When exogenous required nutrient were supplied in vivo , the pathogenicity of glycine requiring mutant was restored; however, the mutant requiring histidine and arginine remained avirulent. No reversion was observed in any of the auxotrophic mutant studied.

ASSUNTO(S)

xanthomanas campestris bacterias fitopatogenicas

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