Estudo morfologico, morfometrico e estereologico da placenta e tecidos fetais, na transmissão transplacentaria de Trypanosoma Cruzi em camundongos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2000

RESUMO

Morphologic alterations of placentas and foetus of Mus musculus infected with RAL strain of Trypanosoma cruzi were investigated by histopathological, morphometrical and stereological methods. On the 7th day of pregnancy, ten female swiss mice, weighing approximately 30-35gr were i.p. inoculated with 2x105 blood trypomastigotes of RAL strain. The animals were sacrified with ethylic ether on the 19th day of pregnancy. Control group (non infected pregnant females) were i.p. inoculated with saline solution and kept in the same enviroment conditions of the infected counterparts. In both experimental groups, weight and lenght of foetus, diameter and weight of placenta as well as umbilical cord lenght were assessed. Histopathology, cariometry and stereology of placentas and fetal tis sues (liver, heart and pancreas) were performed. Cariometry of giant cells and spongyotrophoblasts were assessed too. Infected animals showed reduction of fetal weight and lenght, as well as the diameter and weight of placentas, with enshortened umbilical cord. Histopathologicaly, infected group displayed amastigote burdens in decidua, spongy and labyrinthic zone with scarce inflamatory reaction. Giant cells showed also amastigote burdens with few tissue disorganization. No parasite burdens were found in fetal organs (pancreas,liver and heart).Giant cells of infected animals showed significant reduction of nuclear parameters when compared to control group. The stereological study of placenta from infected animals revealed a spongy region with a smaller relative volume when compared to non infected counterparts although the absolute volume displayed significant differences in all regions of the placenta. The relative cellular and cytoplasmatic volume of giant cells as well as the number / mm3 and the total number / placenta, were significantly lower in infected group whencompared to non infected control animals. We conclude that, the functional alterations on spongioblasts and giant trophoblastic cells caused by RAL strain of T.cruzi can direct1y impair intrauterine fetal development through two distinct ways: directly by depressing production of placental lactogen or indirectly by leading to an inefficient uterus placental vascularization, impairing fetal nutrition

ASSUNTO(S)

placenta tripanossomo feto

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