Effect of a high fat diet on mitochondria: bioenergetics, K+ transport and redox state / Efeito de dietas hiperlipídicas sobre mitocôndrias de fígado de camundongos: bioenergética, transporte de K+ e estudo redox

AUTOR(ES)

Ariel Rodrigues Cardoso

DATA DE PUBLICAÇÃO

2009

RESUMO

Obesity is associated with multiple dysfunctions including dyslipidemia, insulin resistance and diabetes. These malfunctions are associated with modifications in mitochondrial physiology. Furthermore, mitochondria are the most important site of energy metabolism and reactive oxygen species production. Alberici et al. (2006) demonstrated that hypertriglyceridemic transgenic mice display increased expression ad activity of mitochondrial ATP sensitive potassium channels (mitoKATP), a mild uncoupling pathway, in liver. This suggests that mitoKATP is an important adaptation to regulate body mass and metabolism. The aim of this work is to investigate the role of mitoKATP in a high fat diet induced by soy oil supplementation, correlating changes in channel activity with metabolic and mitochondrial parameters. Mitochondria were isolated from mouse livers and serological parameters were measured for each animal. Light scattering (to estimate mitoKATP activity), hydrogen peroxide generation, membrane potentials and oxygen consumption were measured in the mitochondrial suspensions. Our results indicate an increase in body mass, without dyslipidemia; and increases in mitoKATP activity, in time-dependent manner, directly correlated to cholesterol levels. In addition, we found increases in ROS generation and decreased capacity of energy conversion (ADP/O) in the high fat diet group. In conclusion, our results indicate that the activity of mitoKATP could be induced by high fat diets and that this is an novel site for metabolic and body mass control.

ASSUNTO(S)

mitokatp ros mitocôndrias (estudo; metabolismo) bioquímica energy conversion estresse oxidativo mitokatp biochemistry potencial de membrana eros conversão energética obesidade obesity membrane potential mitochondria

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