DinÃmica da IgA secretora especÃfica na lÃgrima empacientes portadores de uveÃte posterior ativa presumivelmente por Toxoplasma gondii durante o primeiro trimestre da doenÃa

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Toxoplasma gondii (T.gondii) is the main cause of posterior uveitis and toxoplasmic retinochoroiditis is considered the most common infection of the retina. Serological tests are important tools for the diagnosis of toxoplasmosis, but in ocular toxoplasmosis, usually there is no correlation between levels of serum antibodies and eye symptoms in the patient. Thus, in the case of ocular toxoplasmosis, the serological tests are of little help. Some studies have shown that the quantification of antibodies in the aqueous humor can be enlightening, however they are tests that require puncture of anterior chamber, this is an invasive procedure. However, other studies have concluded that through the determination of the secretory immunoglobulin A (sIgA) anti-T.gondii in tears, it is possible to differentiate patients with active uveitis caused by toxoplasmosis, from patients with uveitis caused by other etiologies. This study aimed to evaluate the dynamics of the production of anti-sIgA T.gondii in tears in patients with posterior uveitis presumably by T.gondii active during the first quarter of the disease. Sixteen patients who had the classic form of eye disease, or lesions of posterior uveitis with active satellite lesion healed, were divided into two groups at the start of the symptoms: Group I (day 0) and group II (30th). Tears were collected every 15th day and the last sample with 30 days to complete the first quarter. The determination of IgG and IgM serum was done by the immunofluorescence method described by Camargo (1964). To search for tear anti T.gondii sIgA, was used the method described by Lynch et al. (2007) through technique of enzyme immunoassay (ELISA).. All patients had serum IgG positive and serum IgM negative. Except one, all the patients showed positive IgAs at the 15th. day Negativation was observed in few of them around the 60th day. Antibody levels so their profile was individually different although not statistically significant. No statistically significant difference was seen between the ranges studied. There was also reactivity of the healthy eye, which mostly followed the production of sIgA eye patients. We can conclude that the momentum of anti-secretory IgA T.gondii in tears in the first quarter of the active disease, occurs in a variable for each individual

ASSUNTO(S)

lÃgrima immunodiagnosis tear imunodiagnÃstico imunoglobulina a immunoglobulin a ocular toxoplasmosis toxoplasmose ocular medicina

ACESSO AO ARTIGO

Documentos Relacionados