Development of methods to access phospholipase A2 fraction activity in platelets / "Desenvolvimento de métodos para determinação da atividade das frações da fosfolipase A2 em plaquetas"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Phospholipase A2 (PLA2) is a key-enzyme in the metabolism of membrane phospholipids and is one of the major components involved in cell signaling. Alterations of PLA2 activity have been reported in brains and blood cells in several neuropsychiatric diseases. In this study we developed a radio-enzymatic assay to detect in platelets the activity of the three main groups of PLA2, which are secretory PLA2 or extracellular calcium dependent PLA2 (sPLA2), cytosolic calcium dependent PLA2 (cPLA2) and intracellular calcium independent PLA2 (iPLA2). To confirm the presence of these PLA2 groups some variables were tested, such as differences in the preferred fatty acid substrate, calcium dependence, and selective inhibition with Bromoenol lactone (BEL) and Methyl Arachidonyl Fluorophosphonate (MAFP). Our findings demonstrate the presence of the three main groups of PLA2 (sPLA2, cPLA2, and iPLA2) in platelets. In addition, this study is in line with others suggesting that platelets, a typical biological sample, can be used as a peripheral model for neurons.

ASSUNTO(S)

blood platelets phospholipase a fosfolipídeos/metabolismo fosfolipase a phospholipids/metabolism radioimmunoassay/methods radioimunoensaio/métodos plaquetas

ACESSO AO ARTIGO

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