Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)
AUTOR(ES)
Bandeira, Raquel D. C. C., Uekane, Thais M., Cunha, Carolina P. da, Geaquinto, Luths R. O., Cunha, Valnei S., Caixeiro, Janaina M. R., Godoy, Ronoel Luiz O., de la Cruz, Marcus Henrique C.
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2012
RESUMO
A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.
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