Otimização e validação de metodo analitico para a determinação de cloranfenicol em alimentos de origem animal utilizando a tecnica LC-ESI-MS-MS. / Development and validation of analytical method for the determination of chloramphenicol in food of animal origin by LC-ESI-MS-MS.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The main toxic effect of chloramphenicol (CAP) in humans is depression of red blood cell production in bone marrow leading to aplastic anemia and leukemia mainly in children. Thus, in 1994, the use of CAP in animals intended to food production was banned in the European Union (EU) due to the potential risk to human health posed by its residues in food. In the US-FDA (US-Food and Drug Administration), Australia and Brazil also prohibited the use of CAP in food- producing animals destined to the human consumption. In this study, a selective and high detectability method was developed and validated for determining CAP residues in several food matrices (fish, shrimp, poultry, eggs, bovine and swine) by LC-ESI-MS-MS. The samples were extracted in a phosphate solution followed by liquid-liquid extraction with ethyl acetate. The chromatographic separation was performed in a C18 column at room temperature and for quantitation and confirmation of CAP it was used mass spectrometry in tandem. For all the matrices, the analytical curves showed r values and recovery higher than 0.98 and 50%, respectively. The accuracies values lay between 85 and 120% and the precision was lower than 20%. The limit of quantitation was 0.1 ng g-1. The analytical run was accomplished in 6 min.

ASSUNTO(S)

drogas veterinarias chloramphenicol cloranfenicol mass spectrometry food alimentos veterinary drugs espectrometria de massa

ACESSO AO ARTIGO

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