Development and validation of a High Performance Liquid Chromatographic method for determination of etoposide in biodegradable polymeric implants
AUTOR(ES)
Solano, Ana Gabriela Reis, Silva, Gisele Rodrigues da, Fialho, Silvia Ligório, Cunha Júnior, Armando da Silva, Pianetti, Gérson Antônio
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2012
RESUMO
A method using HPLC-UV was developed and validated for the determination of etoposide incorporated into polycaprolactone implants. The method was carried out in isocratic mode using a C18 column (250 x 4.6 mm; 5 µm), at 25 ºC, with acetonitrile and acetic acid 4% (70:30) as mobile phase, a flow rate of 2 mL/min, and UV detection at 285 nm. The method was linear (r² > 0.99) over the range of 5 to 65 µg/mL, precise (RSD < 5%), accurate (recovery of 98.7%), robust, selective regarding excipient of the sample, and had a quantitation limit equal to 1.76 µg/mL. The validated method can be successfully employed for routine quality control analyses.
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