Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants

AUTOR(ES)

Saliba, Juliana Barbosa, Cunha Junior, Armando da Silva, Gomes, Elionai Cassiana de Lima, Mansur, Herman Sander, Silva, Gisele Rodrigues da

FONTE

Química Nova

DATA DE PUBLICAÇÃO

2011

RESUMO

An HPLC method was developed and validated aiming to quantify the cyclosporine-A incorporated into intraocular implants, released from them; and in direct contact with the degradation products of PLGA. The separation was carried out in isocratic mode using acetonitrile/water (70:30) as mobile phase, a C18 column at 80 ºC and UV detection at 210 nm. The method provided selectivity based on resolution among peaks. It was linear over the range of 2.5-40.0 µg/mL. The quantitation and detection limits were 0.8 and 1.2 µg/mL, respectively. The recovery was 101.8% and intra-day and inter-day precision was close to 2%.

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