Desenvolvimento de um biossensor para determinaÃÃo de triglicerÃdeos

AUTOR(ES)

Leonardo Pereira de Siqueira

DATA DE PUBLICAÇÃO

2009

RESUMO

Due to the necessity of more versatile methods for measure and accompany the lipid serum level, the production of several new analytical methods has been stimulated. This work shows a development of a monoenzymatic biosensor for triglycerides detection using Screen-printing technology, in which the reference electrodes (Ag/AgCl) and work electrode containing a mixture of carbon and chitosan 5% (w/w) were printed. The enzyme lipase was immobilized by adsorption on the work electrode. The soluble lipase presented optimum pH of 8.0, temperature of 32.5ÂC, keeping around 78% of its maximum activity at 25ÂC (biosensor work temperature). Km and Vmax for soluble lipase using pNPM as substrate were 0.95 mM and 25.51 U/mg protein, respectively. However for the developed biosensor with immobilized lipase only using triolein as substrate Km and Vmax were 0.148mM and 262.21 μA/s, 0.34 mM and 85.07 μA/s for soluble lipase and TIP without enzyme, respectively. The results showed linearity when triolein was used as substrate (0,25mM â 2mM) certifying the viability of the biosensor developed, using only immobilized lipase on the surface of work electrode, suggesting that it can be efficiently employed in the triglicerides detection

ASSUNTO(S)

triglycerides triglicerÃdeos biossensor eletroquÃmico lipÃdios screen-printing electrochemical biosensors lipase bioquimica lipase

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