Cloning and characterization of glucose-regulated genes in human pancreatic islets / "Clonagem e caracterização de genes regulados por glicose em ilhotas pancreáticas humanas"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Type 1 Diabetes mellitus (T1DM) is caused by autoimmune destruction of the insulin-producing pancreatic islet b-cells. Treatment is generally approached by daily subcutaneous injections of exogenous insulin. Nowadays, pancreatic islet transplantation is considered as an effective alternative treatment to insulin therapy. However, in order to reach insulin-independence, a large number of islets is required for each patient. Knowledge of the mechanisms regulating islet b-cell proliferation may allow ex-vivo b-cell expansion prior to transplant. Glucose is considered one of the main inducers of islet b-cells proliferation. We established and executed the technology of human islet isolation and purification. The islets were then stimulated in culture with glucose. In order to identify glucose-regulated genes in cultured human islets, we utilized the suppression subtractive hybridization (SSH) method, followed by cDNA library screening by DNA macroarrays. Preliminary screening allowed us to isolate two cDNAs displaying glucose regulation, one of which is similar to a human hypothetical protein of unknown function and the other shows similarity to the pancreatic polypeptide receptor. This work allowed identification of glucose-regulated genes in human pancreatic islets, which may be related to cell proliferation in this tissue.

ASSUNTO(S)

clonagem gênica transplante de ilhotas human pancreatic islets beta cell proliferation diabetes mellitus do tipo 1 proliferação de células beta glucose glicose hibridização subtrativa islet transplantation type 1 diabetes mellitus ilhotas pancreáticas humanas suppression subtractive hybridization gene cloning

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