Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
AUTOR(ES)
Aragão, T.C.F.R., Souza, P.A.S., Uchôa, A.F., Costa, I.R., Bloch Jr., C., Campos, F.A.P.
FONTE
Brazilian Journal of Medical and Biological Research
DATA DE PUBLICAÇÃO
2000-08
RESUMO
We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.
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