Avaliação do valor de predição de clivagem química de radicais hidroxila em regiões promotoras de genes ligados ao desenvolvimento craniofacial / Evaluation of predictive value for chemical cleavage of hydroxy radicals in the promoter regions of genes related to craniofacial developmentl

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

28/02/2012

RESUMO

The initiation of the gene transcription is a very complex phenomenon, probably the principal stage where the control of the gene expression takes place. In general terms the transcription takes place with the connection of proteins called "transcription factors" with specific sequences of the DNA, called of regions or sequences "cis ". The interaction DNA - protein can depend on the configuration of the DNA in these sequences. The interaction of the transcription factors with the site in the DNA depends not only on the bases where the contact takes place, but they can depend also on the nearby bases and configuration of DNA. The aim of this work was to investigate the importance of the structural double band configuration of the DNA of conserved sequences in promoter region of genes (between the nucleotídeos-1 and-500), in genes involved in mammalian craniofacial development .We analyzed if the variation in the structure of the DNA caused by genet ic variations in regions phylogenetically preserved differs from the variation in the structure in less preserved regions. The 500 bp sequences of DNA of the regions 5 of the 22 genes, were obtained of the site Ensembl (http: // www.ensembl.org/index.html). We used sequences of the five species of mammals. The structure (secondary) of DNA was estimated by the prediction hydroxyl radical cleavage of the DNA double strand, by the algorithm Orchid (http: // dna.bu.edu/orchid/). The sequences of five selected species were aligned in the program Clustalw (http: // www.ebi.ac.uk/Tools/clustalw2/index.html)and the regions when 0, 1 (conservation of 80 %) and 0, 2 (70 %) with at least 15 bases and without (gaps) were obtained using the program Bioedit (http: // www.mbio.ncsu.edu/bioedit/bioedit.html). To evaluate the predictive value of chemical cleavage, was developed an algorithm in language Ruby version 1.91 (annex), where they obtained values were corresponding to the absolute values of the subtraction of the values of prediction of fracture chemistry of the bases that were differing in the sequences preserved and not preserved between two species. Comparing conserved and not conserved regions, no statistically significant differences in standard chemical cleavage when substituted nucleotides in promoter region.

ASSUNTO(S)

dna dna

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