Avaliação da atividade genotóxica do lapachol e β-lapachona e anticarcinogênica do lapachol, em células somáticas de Drosophila melanogaster

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

This study investigated the genotoxicity of Lapachol (LAP) evaluated by wing spot test of Drosophila melanogaster in the descendants from standard (ST) and high bioactivation (HB) crosses. This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the flys wings. Drosophila has extensive genetic homology to mammals, which makes it a suitable model organism for genotoxic investigations. Larvae of three days were used, resulting from ST cross (females flr3 /TM3, Bds x males mwh/mwh), with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross) (females ORR; flr3/ TM3, Bds x males mwh/mwh). The results showed that LAP is a promutagen, exhibiting genotoxic activity in larvae from the HB cross, in other words, there is an increase in the frequency of spots occurring only in individuals with a high level of the cytochrome P450. Results also indicate that recombinogenicity is the main genotoxic event induced by LAP.

ASSUNTO(S)

drosophila melanogaster doxorubicin doxorrubicina naftoquinonas genetica wing spot test epitélio - câncer cytochrome p450 epitélio câncer tratamento lapachol topoisomerase smart

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